A rapid, novel method for the solid-phase derivatization of IgG antibodies for immune-affinity chromatography
| Autor: | J.J. Marchalonis, D.M. Gersten |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Chromatography
biology Immunology medicine.disease_cause Chromatography Affinity Antibodies Anti-Idiotypic Sepharose Epitopes chemistry.chemical_compound Immune system Antigen Affinity chromatography chemistry Staphylococcus aureus Immunologic Techniques biology.protein medicine Immunology and Allergy Antibody Derivatization Protein A Limulus Test |
| Zdroj: | Journal of Immunological Methods. 24:305-309 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(78)90133-3 |
| Popis: | We show that a solid-phase immune adsorbent can be prepared from rabbit antiserum by isolating the IgG fraction with the A protein of Staphylococcus aureus associated covalently with a Sepharose matrix. The IgG is then coupled to the matrix using the cross-linking agent dimethylsuberimidate. IgG antibody bound in this fashion is in the proper orientation for combination with antigen because association with protein A occurs via the Fc portion of the IgG molecule, thus leaving the combining site of the molecule free to interact with antigen. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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