Expression profiling indicating low selenium-sensitive microRNA levels linked to cell cycle and cell stress response pathways in the CaCo-2 cell line
| Autor: | Nicole C. Roy, Mark McCann, John E. Hesketh, Kunjana Rotjanapun |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway In silico Medicine (miscellaneous) Cell Cycle Proteins Biology medicine.disease_cause Biological pathway Selenium 03 medical and health sciences 0302 clinical medicine Stress Physiological microRNA medicine Humans RNA Messenger Nutrition and Dietetics Cell Cycle Wnt signaling pathway Cell cycle Cell biology body regions Gene expression profiling MicroRNAs 030104 developmental biology Gene Expression Regulation 030220 oncology & carcinogenesis embryonic structures Caco-2 Cells Transcriptome Carcinogenesis |
| Zdroj: | British Journal of Nutrition. 117:1212-1221 |
| ISSN: | 1475-2662 0007-1145 |
| DOI: | 10.1017/s0007114517001143 |
| Popis: | Se is an essential micronutrient for human health, and fluctuations in Se levels and the potential cellular dysfunction associated with it may increase the risk for disease. Although Se has been shown to influence several biological pathways important in health, little is known about the effect of Se on the expression of microRNA (miRNA) molecules regulating these pathways. To explore the potential role of Se-sensitive miRNA in regulating pathways linked with colon cancer, we profiled the expression of 800 miRNA in the CaCo-2 human adenocarcinoma cell line in response to a low-Se (72 h at m) environment using nCounter direct quantification. These data were then examined using a range ofin silicodatabases to identify experimentally validated miRNA–mRNA interactions and the biological pathways involved. We identified ten Se-sensitive miRNA (hsa-miR-93-5p, hsa-miR-106a-5p, hsa-miR-205-5p, hsa-miR-200c-3p, hsa-miR-99b-5p, hsa-miR-302d-3p, hsa-miR-373-3p, hsa-miR-483-3p, hsa-miR-512-5p and hsa-miR-4454), which regulate 3588 mRNA in key pathways such as the cell cycle, the cellular response to stress, and the canonical Wnt/β-catenin, p53 and ERK/MAPK signalling pathways. Our data show that the effects of low Se on biological pathways may, in part, be due to these ten Se-sensitive miRNA. Dysregulation of the cell cycle and of the stress response pathways due to low Se may influence key genes involved in carcinogenesis. |
| Databáze: | OpenAIRE |
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