Proteomics of regenerated tissue in response to a titanium implant with a bioactive surface in a rat tibial defect model

Autor: Viorel Iulian Suica, Gabriel Socol, Emanuel Dragan, Sorin Mihai Croitoru, Livia Elena Sima, Raluca Maria Boteanu, Marioara Chiritoiu, Constantin Vlagioiu, Luminita Ivan, Elena Uyy, Valentina Grumezescu, Florentina Safciuc, Felicia Antohe
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Proteomics
Bone Regeneration
lcsh:Medicine
02 engineering and technology
Mass Spectrometry
Immunological techniques
Biopolymers
Coated Materials
Biocompatible
lcsh:Science
Fracture Healing
Titanium
Microscopy
Multidisciplinary
Chemistry
Prostheses and Implants
021001 nanoscience & nanotechnology
0210 nano-technology
Biocompatibility
Surface Properties
Bioinformatics
chemistry.chemical_element
Proteomic analysis
Bone healing
engineering.material
Prosthesis Design
Dip-coating
Article
03 medical and health sciences
Osseointegration
PEG ratio
Cell Adhesion
Animals
Rats
Wistar
Cell Proliferation
Biological models
Fracture repair
Tibia
lcsh:R
Computational Biology
Mesenchymal Stem Cells
Actins
Rats
030104 developmental biology
Microscopy
Fluorescence
engineering
lcsh:Q
Biopolymer
Implant
Wound healing
Biomedical engineering
Zdroj: Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/s41598-020-75527-2
Popis: Due to their excellent mechanical and biocompatibility properties, titanium-based implants are successfully used as biomedical devices. However, when new bone formation fails for different reasons, impaired fracture healing becomes a clinical problem and affects the patient's quality of life. We aimed to design a new bioactive surface of titanium implants with a synergetic PEG biopolymer-based composition for gradual delivery of growth factors (FGF2, VEGF, and BMP4) during bone healing. The optimal architecture of non-cytotoxic polymeric coatings deposited by dip coating under controlled parameters was assessed both in cultured cells and in a rat tibial defect model (100% viability). Notably, the titanium adsorbed polymer matrix induced an improved healing process when compared with the individual action of each biomolecules. High-performance mass spectrometry analysis demonstrated that recovery after a traumatic event is governed by specific differentially regulated proteins, acting in a coordinated response to the external stimulus. Predicted protein interactions shown by STRING analysis were well organized in hub-based networks related with response to chemical, wound healing and response to stress pathways. The proposed functional polymer coatings of the titanium implants demonstrated the significant improvement of bone healing process after injury.
Databáze: OpenAIRE
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