En-face optical coherence tomography/fluorescence endomicroscopy for minimally invasive imaging using a robotic scanner
| Autor: | Guang-Zhong Yang, Michael Hughes, Adrian Gh. Podoleanu, Adrian Bradu, Haojie Zhang, Christopher J. Payne, Andrew D. Thrapp, Manuel J. Marques, Khushi Vyas, Grigory V. Gelikonov, Petros Giataganas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Diagnostic Imaging
Paper Scanner Materials science Swine 0205 Optical Physics Biomedical Engineering Field of view multimodal imaging Lateral resolution 01 natural sciences Imaging 010309 optics Biomaterials scanning Robotic Surgical Procedures Optical coherence tomography 0903 Biomedical Engineering 0103 physical sciences medicine Endomicroscopy Animals Humans QC355 A fibers endoscopy Image resolution Reproducibility medicine.diagnostic_test Reproducibility of Results Optics Dermis Atomic and Molecular Physics and Optics Sweat Glands Electronic Optical and Magnetic Materials 1113 Opthalmology and Optometry Pulmonary Alveoli biomedical optics Microscopy Fluorescence sense organs fluorescence Tomography Optical Coherence Biomedical engineering |
| Zdroj: | Journal of Biomedical Optics |
| ISSN: | 1083-3668 |
| Popis: | We report a compact rigid instrument capable of delivering en-face optical coherence tomography (OCT) images alongside (epi)-fluorescence endomicroscopy (FEM) images by means of a robotic scanning device. Two working imaging channels are included: one for a one-dimensional scanning, forward-viewing OCT probe and another for a fiber bundle used for the FEM system. The robotic scanning system provides the second axis of scanning for the OCT channel while allowing the field of view (FoV) of the FEM channel to be increased by mosaicking. The OCT channel has resolutions of 25 / 60 μm (axial/lateral) and can provide en-face images with an FoV of 1.6 × 2.7 mm2. The FEM channel has a lateral resolution of better than 8 μm and can generate an FoV of 0.53 × 3.25 mm2 through mosaicking. The reproducibility of the scanning was determined using phantoms to be better than the lateral resolution of the OCT channel. Combined OCT and FEM imaging were validated with ex-vivo ovine and porcine tissues, with the instrument mounted on an arm to ensure constant contact of the probe with the tissue. The OCT imaging system alone was validated for in-vivo human dermal imaging with the handheld instrument. In both cases, the instrument was capable of resolving fine features such as the sweat glands in human dermal tissue and the alveoli in porcine lung tissue. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|