Effect of extender and amino acid supplementation on sperm quality of cooled-preserved Andalusian donkey (Equus asinus) spermatozoa
| Autor: | D. Acha, J.J. Carrasco, Manuel Hidalgo, M.J. Gálvez, V. Gómez-Arrones, Jesús Dorado, R. Calero-Carretero, Isabel Ortiz |
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| Rok vydání: | 2014 |
| Předmět: |
Male
endocrine system Cold storage Semen Biology law.invention Andrology Semen quality fluids and secretions Endocrinology Food Animals law Animals Amino Acids Acrosome Sperm motility urogenital system Extender Equidae General Medicine Spermatozoa Sperm Semen Analysis Sperm Motility Animal Science and Zoology Donkey Semen Preservation |
| Zdroj: | Animal Reproduction Science. 146:79-88 |
| ISSN: | 0378-4320 |
| DOI: | 10.1016/j.anireprosci.2014.02.009 |
| Popis: | The main aim of this study was to evaluate the efficacy of two commercially available liquid stallion semen extenders for the preservation of Andalusian donkey semen at 5°C for up to 72h, and to evaluate the effect of amino acid addition on sperm quality of cooled donkey semen. In addition, this study investigated the effect of seasons on semen characteristics of Andalusian jackasses. Throughout a year, 50 ejaculates were collected from ten adult donkeys and a complete semen evaluation was performed immediately after collection. In Experiment 1, semen samples (n=32) were pooled, divided into two aliquots, and cooled in either Gent(®) A or INRA 96(®). In Experiment 2, pooled semen samples (n=9) were cooled in Gent A(®) supplemented with 0 (as control), 20, 40, or 60mM for each glutamine, proline, or taurine. Fresh semen and chilled samples were assessed for sperm motility, morphology, acrosome integrity, and plasma membrane integrity. Sperm motility variables were greater (P |
| Databáze: | OpenAIRE |
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