Transcriptome analyses of Populus × euramericana clone I-214 leaves exposed to excess zinc
| Autor: | Andrea Andreucci, T. Bracci, Luca Sebastiani, Roberto Tognetti, Gianni Barcaccia, D. Di Baccio, Giulio Galla |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Chlorophyll
Candidate gene Physiology Plant Science Biology Transcriptome Quantitative Trait Heritable Gene Expression Regulation Plant Botany oxidative stress Hyperaccumulator KEGG glutathione heavy metals Blast2GO Gene microarrays Crosses Genetic Oligonucleotide Array Sequence Analysis Genetics Comparative genomics Expressed sequence tag photosynthesis Gene Expression Profiling fungi Biosynthetic Pathways Plant Leaves Zinc Populus |
| Zdroj: | Tree physiology 31 (2011): 1293–1308. doi:10.1093/treephys/tpr106 info:cnr-pdr/source/autori:Daniela Di Baccio 1,5; Giulio Galla 2; Tania Bracci 1; Andrea Andreucci 3; Gianni Barcaccia 2; Roberto Tognetti 4; Luca Sebastiani1/titolo:Transcriptome analyses of Populus x euramericana clone I-214 leaves exposed to excess zinc/doi:10.1093%2Ftreephys%2Ftpr106/rivista:Tree physiology/anno:2011/pagina_da:1293/pagina_a:1308/intervallo_pagine:1293–1308/volume:31 |
| DOI: | 10.1093/treephys/tpr106 |
| Popis: | Zinc (Zn) is an essential element for plant growth and development, but at high levels this metal can become toxic. Hyperaccumulator species are often not suitable for phytoremediation technologies because they need to be fast growing and have high biomass production, such as those of the Populus genus. Comparative genomics studies of poplars subjected to stress conditions such as heavy metal contamination have generated resources useful for improving the annotation of genes and have provided novel insights in the defense/tolerance mechanisms governing adaptation in non-hyperaccumulator plants. Using a microarray-based comparative analysis, we identified functional gene sets that are differentially regulated in the leaves of Populus × euramericana clone I-214 subjected to an excess but sub-lethal dose of Zn (1 mM). Eco-physiological and chemical analyses confirmed the results obtained in previous similar experiments. A total of 3861 expressed sequence tags (ESTs) were differentially expressed and grouped into two distinct libraries of up-regulated (40%) and down-regulated (60%) putative genes. The annotation of genes and gene products according to the Gene Ontology vocabularies was performed using Blast2GO software. The two transcriptome data sets were used to query all known Kyoto Encyclopedia of Genes and Genomes (KEGG) biosynthetic pathways of the genes identified in this study. The most represented molecular functions and biological processes were nucleotide binding and transcription, transport and response to stress and abiotic and biotic stimuli. The chloroplast, mitochondrion and their membrane systems were the cellular components most affected by excess Zn, as well as the photosynthetic, defense, sulfur and glutathione (GSH) metabolic pathways. The most up-regulated genes encoded electron carriers associated with ferrodoxin, the small subunit of ribulose-bisphosphate carboxylase oxygenase, and enzymes involved in GSH metabolism. This study is the most in-depth transcriptome and gene-annotation analysis of a hybrid poplar to date. The results are presented and critically discussed in terms of poplar response/tolerance to Zn stress for the characterization of non-hyperaccumulator phenotypes and the identification of candidate genes in peren nial plants. These genetic findings provide useful information on tree species’ adaptation to metal stress and provide power ful tools for the selection and/or genetic manipulation of stress-tolerant poplar clones. |
| Databáze: | OpenAIRE |
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