Increased functional Na(+)-K+ pump activity in the vasculature of fructose-fed hyperinsulinemic and hypertensive rats
| Autor: | Pongamorn Bunnag, Michael S. Golub, Mark T. Hori, Bernard L. Ormsby, Michael L. Tuck, Morris E. Berger |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Blood Glucose
Male Tail medicine.medical_specialty Physiology medicine.medical_treatment Sodium Muscle Relaxation chemistry.chemical_element Blood Pressure Fructose In Vitro Techniques Rats Sprague-Dawley chemistry.chemical_compound Norepinephrine Internal medicine Hyperinsulinism Internal Medicine medicine Hyperinsulinemia Dietary Carbohydrates Animals Humans Insulin Vasoconstrictor Agents Na+/K+-ATPase Dose-Response Relationship Drug Angiotensin II Body Weight Arteries medicine.disease Acetylcholine Electric Stimulation Rats Femoral Artery Dose–response relationship Endocrinology chemistry Hypertension Potassium Arterial blood Sodium-Potassium-Exchanging ATPase Cardiology and Cardiovascular Medicine |
| Zdroj: | Hypertension research : official journal of the Japanese Society of Hypertension. 21(2) |
| ISSN: | 0916-9636 |
| Popis: | We hypothesized that hyperinsulinemia may alter insulin's ability to stimulate vascular Na+/K(+)-ATPase pump activity and modulate changes in vascular responsiveness associated with hypertension. We measured potassium-induced relaxation as an indicator of Na+/K(+)-ATPase pump activity in isolated femoral arteries from fructose-fed (FF) hyperinsulinemic, Sprague-Dawley rats. FF rats had higher mean arterial blood pressures than did normal diet-fed (NF) rats (FF, 125 +/- 2.2, n = 20, vs. NF, 113.5 +/- 2.5 mmHg, n = 20, p < 0.05) and were hyperinsulinemic (FF, 64 +/- 4 vs. NF, 37 +/- 2, microU/ml insulin, p < 0.01). FF rats were more sensitive to KCl in the Na+/K+ pump bioassay (FF, 0.86 +/- 0.07, n = 21 vs. NF, 1.18 +/- 0.08, n = 17, p < 0.05, expressed as ED50 in mmol/l KCl). Exogenous insulin (100 mU/ml) increased Na+/K+ pump sensitivity in FF rats as compared with a boiled insulin control (insulin 45 +/- 6%, n = 11, vs. control, 11 +/- 7%, n = 13, p < 0.01, expressed as percent increase in sensitivity, i.e., ED50). There were no significant differences in Na+/K+ pump sensitivity between insulin and control responses in the NF animals (insulin 29 +/- 6%, n = 11, vs. control 46 +/- 5%, n = 10, NS). Dose-response curves were obtained in tail and femoral arteries from the same animals to norepinephrine and acetylcholine, basally and after exogenous insulin. FF vessels had reduced sensitivity to norepinephrine as compared with the NF group. Insulin increased sensitivity to acetylcholine-induced relaxations and increased AII-induced contractions in FF-rat vessels. These data suggest that in the FF rat insulin's influence on the vascular Na+/K+ pump is enhanced and may modulate the changes in vascular responsiveness seen in this model. |
| Databáze: | OpenAIRE |
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