Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil
| Autor: | Fabíola Souza Fiaccadori, Menira Souza, Paulo Sucasas da Costa, Ana Maria Tavares Borges, Teresinha Teixeira de Sousa, Divina das Dôres de Paula Cardoso |
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| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Male lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 human bocavirus lcsh:QR1-502 medicine.disease_cause Polymerase Chain Reaction lcsh:Microbiology law.invention Parvoviridae Infections Feces Phylogenetics law Rotavirus Human bocavirus medicine Humans acute gastroenteritis Gene Polymerase chain reaction Phylogeny biology Phylogenetic tree molecular characterisation Acute gastroenteritis biology.organism_classification Virology Gastroenteritis Child Preschool Acute Disease DNA Viral Female Seasons Aichi virus Brazil |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 107, Iss 6, Pp 800-804 (2012) Memórias do Instituto Oswaldo Cruz, Volume: 107, Issue: 6, Pages: 800-804, Published: SEP 2012 |
| ISSN: | 1678-8060 |
| Popis: | To determine the positivity rate of human bocavirus (HBoV) 1 and 3 among children who presented with acute gastroenteritis symptoms during the period of 1994-2004 in the Central-West Region of Brazil, 762 faecal samples were tested using polymerase chain reaction (PCR) for the detection of HBoV DNA. Primers for a segment of the non-structural viral protein 1 (NS1) gene of HBoV-1 and HBoV-3 were used. Twelve HBoV-positive samples were further characterised via genomic sequencing and phylogenetic analysis. Of the samples tested, 5.8% (n = 44) were positive for HBoV-1 or HBoV-3 and co-infection was observed in 14 (31.8%) of the 44 HBoV-positive samples. Nine of the 14 samples were also positive for Rotavirus A and five were positive for Aichi virus. The genomic sequencing of the NS1 partial sequence of 12 HBoV-samples showed that 11 samples were characterised as HBoV-1 and that one was characterised as HBoV-3. The phylogenetic analysis showed that the HBoV-1 samples had a high sequence homology to others previously identified in China, Sweden and Brazil. This is the first study conducted in the Central-West Region of Brazil to detect HBoV-1 and HBoV-3 in faecal samples from children with acute gastroenteritis. Further studies are required to define the role of HBoVs as aetiological agents of gastroenteritis. |
| Databáze: | OpenAIRE |
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