Robotic Real-time Near Infrared Targeted Fluorescence Imaging in a Murine Model of Prostate Cancer: A Feasibility Study
| Autor: | Pedro F. Escobar, Warren D. W. Heston, Steve Huang, Cristina Magi-Galluzzi, Kelley M. Harsch, Sricharan Chalikonda, Georges-Pascal Haber, Robert J. Stein, Xinning Wang, Pravin K. Rao, Rakesh Khanna, Riccardo Autorino, Jihad H. Kaouk, Humberto Laydner, Wahib Isac, Bo Hu |
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| Přispěvatelé: | Laydner, H, Huang, S, Heston, Wd, Autorino, Riccardo, Wang, X, Harsch, Km, Magi Galluzzi, C, Isac, W, Khanna, R, Hu, B, Escobar, P, Chalikonda, S, Rao, Pk, Haber, Gp, Kaouk, Jh, Stein, Rj |
| Rok vydání: | 2013 |
| Předmět: |
Glutamate Carboxypeptidase II
Male Pathology medicine.medical_specialty Fluorescence-lifetime imaging microscopy Infrared Rays Urology Prostate cancer cell Mice SCID urologic and male genital diseases Mice Prostate cancer Mice Inbred NOD Cell Line Tumor medicine Animals Prostate tumors Fluorescent Dyes Membrane antigen Membrane Glycoproteins business.industry Optical Imaging Prostatic Neoplasms Robotics medicine.disease Fluorescent Antibody Technique Direct Murine model Feasibility Studies Signal intensity business Neoplasm Transplantation |
| Zdroj: | Urology. 81:451-457 |
| ISSN: | 0090-4295 |
| Popis: | Objective To evaluate the detection of near-infrared fluorescence from prostate tumors stained with a prostate-specific membrane antigen (PSMA)–targeted tracer developed in our institution with a novel robotic imaging system. Methods Prostate cancer cell lines PC3-pip (PSMA positive) and PC3-flu (PSMA negative) were implanted subcutaneously into 6 immunodeficient mice. When tumors reached 5 mm, a PSMA-targeted fluorescent conjugate was injected intravenously. The first 3 mice underwent near-infrared imaging immediately and hourly up to 4 hours after injection to determine the time necessary to obtain peak fluorescence and were killed. The last 3 mice were imaged once preoperatively and were euthanized 120 minutes later. Excision of the tumors was performed by using a novel robotic imaging system to detect near-infrared fluorescence in real time. Specimens were submitted for pathology. Results In the first 3 mice, we found 120 minutes as the time needed to observe peak fluorescence from the PSMA-positive tumors. We identified discrete near-infrared fluorescence from 2 of 3 PSMA-positive tumors with the robotic imaging system. Surgical margins were negative for all excised specimens except for one PSMA-negative tumor. Conclusions Real-time near-infrared fluorescence imaging of prostate cancer is feasible with a novel robotic imaging system. Further research is needed to optimize the signal intensity detectable from prostate cancer with our tracer. Toxicologic studies are needed before its clinical use. |
| Databáze: | OpenAIRE |
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