Haemonchus contortus β-tubulin isotype 1 gene F200Y and F167Y SNPs are both selected by ivermectin and oxfendazole treatments with differing impacts on anthelmintic resistance
| Autor: | Marcel Teixeira, Luiz da Silva Vieira, Claudia Maria Leal Bevilaqua, Wesley Lyeverton Correia Ribeiro, Weibson Paz Pinheiro Andre, Jessica Maria Leite dos Santos, J. P. Monteiro, G. A. Frota, J. F. Vasconcelos |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Veterinary medicine Oxfendazole Drug Resistance Sheep Diseases Single-nucleotide polymorphism Pharmacology Polymorphism Single Nucleotide 03 medical and health sciences Ivermectin Tubulin parasitic diseases medicine Animals Anthelmintic Selection Genetic Gene Larva Sheep General Veterinary biology Antinematodal Agents General Medicine 030108 mycology & parasitology biology.organism_classification Isotype Benzimidazoles Haemonchus Parasitology Haemonchiasis Haemonchus contortus medicine.drug |
| Zdroj: | Veterinary Parasitology. 248:90-95 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/j.vetpar.2017.11.003 |
| Popis: | Parasitism by Haemonchus contortus is one of the main limiting factors in small ruminant production in tropical areas. Benzimidazoles (BZ) and macrocyclic lactones (ML) are the most used anthelmintic classes in gastrointestinal nematodes control. There is considerable scientific evidence of a possible relation between the anthelmintic resistance to BZ and ML. This study aimed to characterize the dynamics of anthelmintic resistance in an H. contortus susceptible isolate under selection pressure for BZ and ML alone or in combination and the role of isotype 1 β-tubulin gene SNPs in these situations. A total of 12 Somali sheep were infected with 5000 third stage larvae of H. contortus Inbred-Susceptible Edinburgh (ISE) isolate. Once infection was established, animals were distributed in three groups (n=4), each treated with crescent doses of oxfendazole (OXF), ivermectin (IVM) and oxfendazole plus ivermectin (IVMOXF). An additional control group with untreated animals was maintained during the entire experiment. After each treatment, eggs were collected and real-time PCR was performed to identify single nucleotide polymorphisms (SNPs) F167Y, F200Y and E198A, in addition to egg hatch test (EHT) for BZ and larval development test (LDT) for ivermectin resistance. All treatments led to increased resistance allelic frequencies at SNPs F200Y and F167Y (p |
| Databáze: | OpenAIRE |
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