Characterization of Melanogenesis Inhibitory Constituents of Morus alba Leaves and Optimization of Extraction Conditions Using Response Surface Methodology
| Autor: | Yang Hee Jo, Ji Yeon Jeong, Seon Beom Kim, Hyo Hee Yang, Dae Hye Song, Bang Yeon Hwang, Eun Jin Mo, Qing Liu, Mi Kyeong Lee |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
melanogenesis
Chalcone Tyrosinase Pharmaceutical Science Biology Stilbenoid tyrosinase Article Analytical Chemistry Melanin lcsh:QD241-441 Pigment chemistry.chemical_compound lcsh:Organic chemistry Phenols Hyperpigmentation Cell Line Tumor Drug Discovery Botany Stilbenes medicine Humans Physical and Theoretical Chemistry Melanoma Benzofurans Flavonoids Melanins Traditional medicine Monophenol Monooxygenase Plant Extracts Organic Chemistry Morus alba Moraceae biology.organism_classification melanin Plant Leaves chemistry Chemistry (miscellaneous) Skin hyperpigmentation visual_art visual_art.visual_art_medium phenolic content Molecular Medicine Morus medicine.symptom optimization |
| Zdroj: | Molecules Molecules, Vol 20, Iss 5, Pp 8730-8741 (2015) Volume 20 Issue 5 Pages 8730-8741 |
| ISSN: | 1420-3049 |
| Popis: | Melanin is a natural pigment that plays an important role in the protection of skin, however, hyperpigmentation cause by excessive levels of melatonin is associated with several problems. Therefore, melanogenesis inhibitory natural products have been developed by the cosmetic industry as skin medications. The leaves of Morus alba (Moraceae) have been reported to inhibit melanogenesis, therefore, characterization of the melanogenesis inhibitory constituents of M. alba leaves was attempted in this study. Twenty compounds including eight benzofurans, 10 flavonoids, one stilbenoid and one chalcone were isolated from M. alba leaves and these phenolic constituents were shown to significantly inhibit tyrosinase activity and melanin content in B6F10 melanoma cells. To maximize the melanogenesis inhibitory activity and active phenolic contents, optimized M. alba leave extraction conditions were predicted using response surface methodology as a methanol concentration of 85.2% an extraction temperature of 53.2 °C and an extraction time of 2 h. The tyrosinase inhibition and total phenolic content under optimal conditions were found to be 74.8% inhibition and 24.8 μg GAE/mg extract, which were well-matched with the predicted values of 75.0% inhibition and 23.8 μg GAE/mg extract. These results shall provide useful information about melanogenesis inhibitory constituents and optimized extracts from M. alba leaves as cosmetic therapeutics to reduce skin hyperpigmentation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|