Suitability of anodic stripping voltammetry for routine analysis of venous blood from raptors
| Autor: | Octavio P. Luzardo, Natalia Pastor Tiburón, Manuel Luis Zumbado Peña, María Camacho, Fernando González, Luis Revuelta Rueda |
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| Rok vydání: | 2018 |
| Předmět: |
040301 veterinary sciences
Health Toxicology and Mutagenesis 010501 environmental sciences 01 natural sciences Sensitivity and Specificity 0403 veterinary science Food and drug administration Animal science Environmental Chemistry Medicine Animals Humans Routine analysis Electrodes 0105 earth and related environmental sciences Whole blood Detection limit Raptors business.industry Limits of agreement 04 agricultural and veterinary sciences Venous blood Predictive value Anodic stripping voltammetry Lead Environmental Pollutants business Blood Chemical Analysis Environmental Monitoring |
| Zdroj: | Environmental toxicology and chemistry. 38(4) |
| ISSN: | 1552-8618 |
| Popis: | Lead (Pb) poisoning is a significant threat faced by raptors. Hence, rapid Pb diagnosis has become a priority during the admission of raptors in wildlife recovery centers, and bench-top analyzers, such as LeadCare II ®, are routinely employed for this purpose. However, this device has been designed for conducting analyses of human blood Pb levels (BLLs), and the validity of this methodology for whole blood from raptors has, to date, rarely been assessed. In addition, a recent recall by the US Food and Drug Administration has recommended discontinuing the use of this analyzer for human venous blood because it may underestimate the BLL. We evaluated the precision of BLL measurements taken with LeadCare II by comparing them with those obtained with inductively coupled plasma mass spectrometry (ICP-MS). Our sample contained venous blood from 105 raptors belonging to 4 species. The results showed a good correlation between the 2 techniques (Spearman's r = 0.927, p < 0.0001). The mean BLL with ICP-MS was 19.6 μg/dL; it was found to be 18.7 μg/dL with LeadCare II. A Bland-Altman analysis indicated that the bias between the mean differences was only 0.5 μg/dL, but it had a high standard deviation of bias (5.7 μg/dL) and 95% limits of agreement from -10.75 to 11.74 μg/dL. The present results indicated that LeadCare II has an overall sensitivity of 71.8% and a positive predictive value of 76.3%. The specificity of LeadCare II for detecting animals with low BLL ( |
| Databáze: | OpenAIRE |
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