| Autor: |
Freire-Pritchett, P, Schoenfelder, S, Várnai, C, Wingett, SW, Cairns, J, Collier, AJ, García-Vílchez, R, Furlan-Magaril, M, Osborne, CS, Fraser, P, Rugg-Gunn, PJ, Spivakov, M |
| Přispěvatelé: |
Schoenfelder, Stefan [0000-0002-3200-8133], Rugg-Gunn, Peter [0000-0002-9601-5949], Apollo - University of Cambridge Repository |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| DOI: |
10.17863/cam.10468 |
| Popis: |
Long-range $\textit{cis}$-regulatory elements such as enhancers coordinate cell-specific transcriptional programmes by engaging in DNA looping interactions with target promoters. Deciphering the interplay between the promoter connectivity and activity of $\textit{cis}$-regulatory elements during lineage commitment is crucial for understanding developmental transcriptional control. Here, we use Promoter Capture Hi-C to generate a high-resolution atlas of chromosomal interactions involving ~22,000 gene promoters in human pluripotent and lineage-committed cells, identifying putative target genes for known and predicted enhancer elements. We reveal extensive dynamics of $\textit{cis}$-regulatory contacts upon lineage commitment, including the acquisition and loss of promoter interactions. This spatial rewiring occurs preferentially with predicted changes in the activity of $\textit{cis}$-regulatory elements and is associated with changes in target gene expression. Our results provide a global and integrated view of promoter interactome dynamics during lineage commitment of human pluripotent cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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