RGD peptide-assisted vitrectomy to facilitate induction of a posterior vitreous detachment: a new principle in pharmacological vitreolysis
| Autor: | Misako Tatebayashi, David L. Epstein, Leonardo B. Oliveira, Janardan Kumar, Cynthia A. Toth, Brooks W. McCuen, Carsten H. Meyer, Fulton Wong |
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| Rok vydání: | 2003 |
| Předmět: |
Pars plana
medicine.medical_specialty genetic structures medicine.medical_treatment Vitrectomy Apoptosis Vitreous Detachment Posterior vitreous detachment Cellular and Molecular Neuroscience chemistry.chemical_compound Animal model Ophthalmology medicine In Situ Nick-End Labeling Animals Ultrasonography RGE peptide Phosphate buffered saline RGD peptide Retinal medicine.disease eye diseases Sensory Systems Surgery medicine.anatomical_structure chemistry Microscopy Electron Scanning sense organs Rabbits Oligopeptides |
| Zdroj: | Current eye research. 25(6) |
| ISSN: | 0271-3683 |
| Popis: | To evaluate a new concept in pharmacological vitreolysis by studying the efficacy of intravitreal RGD peptide-assisted vitrectomy in facilitating the separation of the posterior cortical vitreous from the retinal surface in an animal model.Eight rabbits (16 eyes) received an intravitreal injection of 1 or 5 mg of RGD peptide in one eye and either RGE peptide (inactive control) or phosphate buffered saline in the fellow eye. After 24 hours, a pars plana vitrectomy with low aspiration (or =30 mmHg) was performed in an attempt to create a detachment of the posterior cortical vitreous. A masked observer performed pre- and postoperative indirect ophthalmoscopy and B-scan ultrasonography. Postoperative scanning electron microscopy evaluated the vitreoretinal surface in selected eyes. Two additional rabbits received intravitreal injections of RGD peptide in one eye (1 mg and 5 mg) and 1 mg of RGE peptide in the fellow eye to examine apoptosis of the retinal cells by TUNEL assay.Based on postoperative ultrasound findings, six of the eight rabbits had a greater degree of posterior vitreous detachment in the RGD eye compared to the fellow eye (p = 0.03). The total number and the average number of detached quadrants in the group of RGD peptide eyes was twenty-three and 2.85 respectively compared to seven and 0.85 for the control fellow eyes (p = 0.02). Scanning electron microscopy confirmed the presence of postoperative posterior vitreous detachment. There was no evidence of retinal cell apoptosis in RGD injected eyes.RGD peptide-assisted vitrectomy facilitated posterior vitreous detachment in rabbit eyes, suggesting that RGD-containing peptides may prove to be effective adjuncts in producing posterior vitreous separation during vitreous surgery. |
| Databáze: | OpenAIRE |
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