Analysis and evaluation of (neuro)peptides in honey bees exposed to pesticides in field conditions
| Autor: | Amadeo R. Fernández-Alba, María Dolores Gil García, María del Mar Gómez-Ramos, M. Martínez Galera, María José Gómez Ramos |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Health Toxicology and Mutagenesis 010501 environmental sciences Biology Toxicology 01 natural sciences 03 medical and health sciences Honey Bees Pollinator Animals Food science Pesticides Pollination 0105 earth and related environmental sciences High concentration fungi Neuropeptides General Medicine Honey bee Honey Pesticide Bees Pollution Triple quadrupole mass spectrometer 030104 developmental biology behavior and behavior mechanisms Extraction methods Field conditions Chromatography Liquid Environmental Monitoring |
| Zdroj: | Environmental pollution (Barking, Essex : 1987). 235 |
| ISSN: | 1873-6424 |
| Popis: | During the last years, declines in honey bee colonies are being registered worldwide. Cholinergic pesticides and their extensive use have been correlated to the decline of pollinators and there is evidence that pesticides act as neuroendocrine disruptors affecting the metabolism of neuropeptides. However, there is a big absence of studies with quantitative results correlating the effect of pesticide exposure with changes on neuropeptides insects, and most of them are conducted under laboratory conditions, typically with individual active ingredients. In this study, we present an analytical workflow to evaluate pesticide effects on honey bees through the analysis of (neuro)peptides. The workflow consists of a rapid extraction method and liquid chromatography with triple quadrupole for preselected neuropeptides. For non-target analysis, high resolution mass spectrometry, multivariate analysis and automatic identification of discriminated peptides using a specific software and protein sequence databases. The analytical method was applied to the analysis of target and non-target (neuro)peptides in honey bees with low and high content of a wide range of pesticides to which have been exposed in field conditions. Our findings show that the identification frequency of target neuropeptides decreases significantly in honey bees with high concentration of pesticides (pesticide concentrations ≥ 500 μg kg−1) in comparison with the honey bees with low content of pesticides (pesticide concentrations ≤ 20 μg kg−1). Moreover, the principal component analysis in non-target search shows a clear distinction between peptide concentration in honey bees with high level of pesticides and honey bees with low level. The use of high resolution mass spectrometry has allowed the identification of 25 non-redundant peptides responsible for discrimination between the two groups, derived from 18 precursor proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect