Redox-sensitive bZIP68 plays a role in balancing stress tolerance with growth in Arabidopsis
| Autor: | Wuzhen Liu, Huan Zhong, Yiji Xia, Hai Lei Zhang, Yimin Li |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Chromatin Immunoprecipitation Mutant Arabidopsis Plant Science Oxidative phosphorylation Biology medicine.disease_cause 01 natural sciences Transcriptome 03 medical and health sciences Cytosol Gene Expression Regulation Plant Genetics medicine Cysteine Promoter Regions Genetic Transcription factor Cell Nucleus Binding Sites Arabidopsis Proteins Cell Biology Hydrogen Peroxide Biotic stress biology.organism_classification Plants Genetically Modified Cell biology Oxidative Stress 030104 developmental biology Seedlings Mutation Trans-Activators Chromatin immunoprecipitation Oxidation-Reduction Oxidative stress 010606 plant biology & botany Transcription Factors |
| Zdroj: | The Plant journal : for cell and molecular biologyReferences. 100(4) |
| ISSN: | 1365-313X |
| Popis: | Perturbation of the cellular redox state by stress conditions is sensed by redox-sensitive proteins so that the cell can physiologically respond to stressors. However, the mechanisms linking sensing to response remain poorly understood in plants. Here we report that the transcription factor bZIP68 underwent in vivo oxidation in Arabidopsis cells under oxidative stress which is dependent on its redox-sensitive Cys320 residue. bZIP68 is primarily localized to the nucleus under normal growth conditions in Arabidopsis seedlings. Oxidative stress reduces its accumulation in the nucleus and increases its cytosolic localization. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) revealed that bZIP68 primarily binds to promoter regions containing the core G-box (CACGTG) or G-box-like motif of the genes involved in abiotic and biotic stress responses, photosynthesis, biosynthetic processes, and transcriptional regulation. The bzip68 mutant displayed slower growth under normal conditions but enhanced tolerance to oxidative stress. The results from the ChIP-seq and phenotypic and transcriptome comparison between the bzip68 mutant and wildtype indicate that bZIP68 normally suppresses expression of stress tolerance genes and promotes expression of growth-related genes, whereas its inactivation enhances stress tolerance but suppresses growth. bZIP68 might balance stress tolerance with growth through the extent of its oxidative inactivation according to the environment. |
| Databáze: | OpenAIRE |
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