Evidence That the Mechanism of Prenatal Germ Cell Death in the Mouse Is Apoptosis
| Autor: | Catherine Carswell-Crumpton, Edward G. Spack, Electra C. Coucouvanis, Steven W. Sherwood, Patricia P. Jones |
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| Rok vydání: | 1993 |
| Předmět: |
Male
endocrine system Programmed cell death Light Apoptosis Gestational Age Biology Mice Oogonia Fluorescence microscope medicine Animals Scattering Radiation Mice Inbred BALB C Cell Cycle Embryogenesis Embryo Cell Biology Cell sorting Flow Cytometry Spermatogonia Cell biology medicine.anatomical_structure Cell culture Immunology Oocytes Female Germ cell |
| Zdroj: | Experimental Cell Research. 209:238-247 |
| ISSN: | 0014-4827 |
| Popis: | Using fluorescence-activated cell sorting combined with fluorescence microscopy the mechanism of embryonic germ cell death in the mouse has been shown to be apoptosis. Primordial germ cells (PGCs) from embryos at specific developmental stages have been analyzed, and cells with apoptotic morphology have been isolated by cell sorting. In the female, apoptotic oogonia at Day 13 and apoptotic oocytes at Days 15 and 17 were found. In the male, apoptotic cells were seen on Day 13 through Day 17. Apoptotic germ cells were not detected at Day 12 (combined male and female PGCs). Examination of sorted cells by fluorescence microscopy and by light microscopic analysis after alkaline phosphatase staining confirmed that the cells are apoptotic germ cells. Electron microscopy further confirmed that cells showing the morphological characteristics of apoptosis are present. |
| Databáze: | OpenAIRE |
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