Adenoviral gene transfer of a single‐chain IL‐23 induces psoriatic arthritis–like symptoms in NOD mice
| Autor: | Christopher H. Evans, Jing Zhao, Consuelo M. Lopez De Padilla, Matthew J. Yousefzadeh, Montina Van Meter, Nam Vo, Lei Zhang, Lana Carbo, Andrea Gambotto, Enrico Pola, Luise A. Angelini, Laura J. Niedernhofer, Eun Kim, Debora Colangelo, Paul D. Robbins, Rafael Flores |
|---|---|
| Přispěvatelé: | Flores, Rafael R, Carbo, Lana, Kim, Eun, Van Meter, Montina, De Padilla, Consuelo M Lopez, Zhao, Jing, Colangelo, Debora, Yousefzadeh, Matthew J, Angelini, Luise A, Zhang, Lei, Pola, Enrico, Vo, Nam, Evans, Christopher H, Gambotto, Andrea, Niedernhofer, Laura J, Robbins, Paul D |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Erythema adenoviru type 1 diabetes Enzyme-Linked Immunosorbent Assay adenovirus interleukin-17 interleukin-23 psoriasis Biochemistry Adenoviridae Viral vector Mice 03 medical and health sciences Psoriatic arthritis 0302 clinical medicine Settore MED/04 - PATOLOGIA GENERALE Mice Inbred NOD Psoriasis Genetics medicine Interleukin 23 Animals Molecular Biology Skin psoriasi NOD mice Inflammation biology business.industry Research Arthritis Psoriatic Flow Cytometry medicine.disease Immunohistochemistry 030104 developmental biology Immunology biology.protein Female Lymph Nodes Interleukin 17 medicine.symptom Antibody business 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | FASEB J |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fj.201900420r |
| Popis: | Previously, we demonstrated that intratumoral delivery of adenoviral vector encoding single-chain (sc)IL-23 (Ad.scIL-23) was able to induce systemic antitumor immunity. Here, we examined the role of IL-23 in diabetes in nonobese diabetic mice. Intravenous delivery of Ad.scIL-23 did not accelerate the onset of hyperglycemia but instead resulted in the development of psoriatic arthritis. Ad.scIL-23-treated mice developed erythema, scales, and thickening of the skin, as well as intervertebral disc degeneration and extensive synovial hypertrophy and loss of articular cartilage in the knees. Immunological analysis revealed activation of conventional T helper type 17 cells and IL-17-producing gamma delta T cells along with a significant depletion and suppression of T cells in the pancreatic lymph nodes. Furthermore, treatment with anti-IL-17 antibody reduced joint and skin psoriatic arthritis pathologies. Thus, these Ad.scIL-23-treated mice represent a physiologically relevant model of psoriatic arthritis for understanding disease progression and for testing therapeutic approaches.-Flores, R. R., Carbo, L., Kim, E., Van Meter, M., De Padilla, C. M. L., Zhao, J., Colangelo, D., Yousefzadeh, M. J., Angelini, L. A., Zhang, L., Pola, E., Vo, N., Evans, C. H., Gambotto, A., Niedernhofer, L. J., Robbins, P. D. Adenoviral gene transfer of a single-chain IL-23 induces psoriatic arthritis-like symptoms in NOD mice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text