The lysine-rich motif of intrinsically disordered stress protein CDeT11-24 from Craterostigma plantagineum is responsible for phosphatidic acid binding and protection of enzymes from damaging effects caused by desiccation
| Autor: | Jan Petersen, Dorothea Bartels, Horst Röhrig, Steffen Pierog, Sylvia K. Eriksson, Thomas Colby, Pia Harryson |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Physiology
desiccation tolerance Lysine ved/biology.organism_classification_rank.species Amino Acid Motifs Phosphatidic Acids Resurrection plant Plant Science Citrate (si)-Synthase molecular shield Models Biological Desiccation tolerance chemistry.chemical_compound lipid binding Citrate synthase Amino Acid Sequence Desiccation Peptide sequence Enzyme Assays Plant Proteins biology L-Lactate Dehydrogenase ved/biology Phosphatidic acid binding Water Phosphatidic acid Recombinant Proteins Plant Leaves K-segment Biochemistry chemistry Craterostigma biology.protein Mutagenesis Site-Directed Sequence Alignment Research Paper Signal Transduction |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | The late embryogenesis abundant (LEA)-like protein CDeT11-24 is one of the major desiccation-related phosphoproteins of the resurrection plant Craterostigma plantagineum. In this study, it was shown that CDeT11-24 is mostly intrinsically disordered and protects two different enzymes, citrate synthase and lactate dehydrogenase, against damaging effects caused by desiccation. Lipid-binding assays revealed that CDeT11-24 is able to interact with phosphatidic acid, although electrostatic repulsion was expected due to the overall negative net charge of the protein under the tested physiological conditions. CDeT11-24 carries an N-terminal lysine-rich sequence, which is predicted to form an amphipathic α-helix. Analysis of the truncated CDeT11-24 protein identified this region to be responsible for both activities: enzyme protection and phosphatidic acid interaction. Possible functions of the CDeT11-24 protein are discussed in the context of desiccation tolerance. Abbreviations: ABA abscisic acid; CD circular dichroism; CS citrate synthase; IDP intrinsically disordered protein; LDH lactate dehydrogenase; LEA late embryogenesis abundant; PA phosphatidic acid; PC phosphatidylcholine; TFE trifluoroethanol. |
| Databáze: | OpenAIRE |
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