Difference in Biological Effects Between Insulin-Like Growth Factor Binding Protein 1 and 3
| Autor: | A. Golde, C. Blat, L. Harel, S. L. S. Drop, J. Villaudy |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Clinical Biochemistry Stimulation CHO Cells Chick Embryo Biology Insulin-like growth factor-binding protein law.invention chemistry.chemical_compound Endocrinology Insulin-Like Growth Factor II Transforming Growth Factor beta law Cricetinae Internal medicine medicine Animals Humans Insulin-Like Growth Factor I Fibroblast Cells Cultured Platelet-Derived Growth Factor DNA synthesis Growth factor Embryo DNA Cell Biology Fibroblasts Insulin-Like Growth Factor Binding Protein 1 Insulin-Like Growth Factor Binding Proteins Blood medicine.anatomical_structure chemistry Recombinant DNA biology.protein Fibroblast Growth Factor 2 Growth inhibition Carrier Proteins hormones hormone substitutes and hormone antagonists |
| Zdroj: | Growth Factors. 10:107-114 |
| ISSN: | 1029-2292 0897-7194 |
| DOI: | 10.3109/08977199409010984 |
| Popis: | Insulin-like growth factor binding proteins 1 and 3 are essentially known as regulators of IGF bioactivity. However, we previously showed that IGFBP-3 was able, in chick embryo fibroblast (CEF), to 100% inhibit DNA synthesis stimulated by calf serum, while the maximal inhibition found with IGFBP-1 was 60%, suggesting a difference between the two IGFBPs in their biological functions. Results of the present work agree with this assumption: (a) Recombinant human IGFBP-3, like rat IGFBP-3, was able to 100% inhibit DNA synthesis stimulation induced by human serum, while this stimulation was 75% decreased by IGFBP-1. However, the most striking difference was observed when the effects of the two IGFBPs were compared for stimulation induced by a serum growth factor (SGF) fraction depleted in IGFs. Stimulation induced by the SFG fraction was more significantly decreased (p0.001) by IGFBP-3 than by IGFBP-1. The mean percent inhibition +/- SEM was 67.1 +/- 2.5 in the presence of IGFBP-3 (200 ng/ml) and 29.3 +/- 2.7 and 34.2 +/- 4 in the presence of 200 and 400 ng/ml IGFBP-1 respectively. Inhibition by 200 ng/ml IGFBP-1 and inhibition by 6 ng/ml IGFBP-3 were additive. However, inhibition by IGFBP-3 and that by IGFBP-1 were no longer additive at high concentrations of IGFBP-3, which might thus replace IGFBP-1. (b) FGF stimulation of CEF was similarly inhibition (65% and 70%) by IGFBP-1 and IGFBP-3. (c) TGF beta stimulation of CEF was more strongly decreased by IGFBP-3 (90%) than by IGFB-1 (60%).(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|