Cell sex affects extracellular matrix protein expression and proliferation of smooth muscle progenitor cells derived from human pluripotent stem cells
| Autor: | Morgaine Green, Bertha Chen, Yanhui Li, Yi Wei, Yan Wen, Fan Zhang, Thomas M. Baer, Prachi Wani, Elise K. Cabral |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
Pluripotent Stem Cells 0301 basic medicine Cellular differentiation Pluripotent stem cell Medicine (miscellaneous) Embryoid body 030204 cardiovascular system & hematology Biology Biochemistry Genetics and Molecular Biology (miscellaneous) lcsh:Biochemistry 03 medical and health sciences 0302 clinical medicine Sex differences Smooth muscle progenitor cell Humans lcsh:QD415-436 Progenitor cell Cell potency Cell proliferation Extracellular Matrix Proteins Sex Characteristics Induced stem cells lcsh:R5-920 Research Cell Differentiation Myoblasts Smooth Muscle Cell Biology Extracellular matrix Embryonic stem cell Estrogen Cell biology 030104 developmental biology Gene Expression Regulation Molecular Medicine Female Stem cell lcsh:Medicine (General) Adult stem cell |
| Zdroj: | Stem Cell Research & Therapy, Vol 8, Iss 1, Pp 1-16 (2017) Stem Cell Research & Therapy |
| ISSN: | 1757-6512 |
| DOI: | 10.1186/s13287-017-0606-2 |
| Popis: | Background Smooth muscle progenitor cells (pSMCs) differentiated from human pluripotent stem cells (hPSCs) hold great promise for treating diseases or degenerative conditions involving smooth muscle pathologies. However, the therapeutic potential of pSMCs derived from men and women may be very different. Cell sex can exert a profound impact on the differentiation process of stem cells into somatic cells. In spite of advances in translation of stem cell technologies, the role of cell sex and the effect of sex hormones on the differentiation towards mesenchymal lineage pSMCs remain largely unexplored. Methods Using a standard differentiation protocol, two human embryonic stem cell lines (one male line and one female line) and three induced pluripotent stem cell lines (one male line and two female lines) were differentiated into pSMCs. We examined differences in the differentiation of male and female hPSCs into pSMCs, and investigated the effect of 17β-estradiol (E2) on the extracellular matrix (ECM) metabolisms and cell proliferation rates of the pSMCs. Statistical analyses were performed by using Student’s t test or two-way ANOVA, p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|