Serological investigation and genotyping of Mycobacterium avium subsp. paratuberculosis in sheep and goats in Inner Mongolia, China

Autor: Li-Feng Wang, Zhan-Sheng Zhang, Yu Wang, Hui-Xin Cheng, Siguo Liu, Guanghui Dang, Shu-Ying Liu, Jinling Wang, Yulin Ding, Li Zhao, Wei-Hong Zhao, Hai-Liang Chai, Liu Yonghong, Yi-Min Ma, Jirintai Sulijid, Fenglong Wang, Zengqiang Miao
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Veterinary medicine
Epidemiology
Paratuberculosis
Artificial Gene Amplification and Extension
Polymerase Chain Reaction
law.invention
Serology
Animal Diseases
Geographical Locations
law
Medicine and Health Sciences
Polymerase chain reaction
Mammals
Multidisciplinary
Database and informatics methods
Goats
Sequence analysis
Eukaryota
Ruminants
Mycobacterium avium subspecies paratuberculosis
Vertebrates
Medicine
Antibody
medicine.symptom
Research Article
China
Asia
Genotype
Bioinformatics
Science
Sheep Diseases
Enzyme-Linked Immunosorbent Assay
Biology
Disease Surveillance
Research and Analysis Methods
medicine
Animals
Molecular Biology Techniques
Genotyping
Molecular Biology
BLAST algorithm
Sheep
Goat Diseases
Gene Mapping
Organisms
Biology and Life Sciences
biology.organism_classification
medicine.disease
People and Places
Amniotes
biology.protein
Herd
Emaciation
Zoology
Mycobacterium avium
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 9, p e0256628 (2021)
ISSN: 1932-6203
Popis: Paratuberculosis a contagious and chronic disease in domestic and wild ruminants, is caused by Mycobacterium avium subspecies paratuberculosis (MAP). Typical clinical signs include intractable diarrhea, progressive emaciation, proliferative enteropathy, and mesenteric lymphadenitis. Paratuberculosis is endemic to many parts of the world and responsible for considerable economic losses. In this study, different types of paratuberculosis and MAP in sheep and goats were investigated in Inner Mongolia, a northern province in China contiguous with two countries and eight other provinces. A total of 4434 serum samples were collected from six cities in the western, central, and eastern regions of Inner Mongolia and analyzed using the ELISA test. In addition, tissue samples were collected from seven animals that were suspected to be infected with MAP. Finally, these tissues samples were analyzed by histopathological examination followed by polymerase chain reaction (PCR), IS1311 PCR-restriction enzyme analysis (PCR-REA), and a sequence analysis of five genes. Among all 4434 ruminant serum samples collected from the six cities in the western, central, and eastern regions of Inner Mongolia, 7.60% (337/4434) measured positive for the MAP antibody. The proportions of positive MAP antibody results for serum samples collected in the western, central, and eastern regions were 5.10% (105/2058), 6.63% (85/1282), and 13.44% (147/1094), respectively. For the seven suspected infected animals selected from the herd with the highest rate of positivity, the gross pathology and histopathology of the necropsied animals were found to be consistent with the pathological features of paratuberculosis. The PCR analysis further confirmed the diagnosis of paratuberculosis. The rest of the results demonstrated that herds of sheep and goats in Inner Mongolia were infected with both MAP type II and type III. To the best of our knowledge, this is the first study of the two subtypes of MAP strains in sheep and goats in Inner Mongolia.
Databáze: OpenAIRE
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