Detection and identification of five common internal grain insect pests by multiplex PCR
| Autor: | Nuria Agustí, Jordi Riudavets, Mireia Solà |
|---|---|
| Přispěvatelé: | Producció Vegetal, Protecció Vegetal Sostenible |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine media_common.quotation_subject Insect 01 natural sciences 03 medical and health sciences Sitotroga cerealella Multiplex polymerase chain reaction medicine media_common biology business.industry Sitophilus 632 - Malalties i protecció de les plantes Contamination biology.organism_classification Food safety Biotechnology 010602 entomology medicine.drug_formulation_ingredient 030104 developmental biology PEST analysis Food quality business Food Science |
| Zdroj: | IRTA Pubpro. Open Digital Archive Institut de Recerca i Tecnologia Agroalimentàries (IRTA) |
| ISSN: | 0956-7135 |
| DOI: | 10.1016/j.foodcont.2017.08.002 |
| Popis: | Consumer demands for better quality food have led to research on new tools aimed at early detection of insect pests in agro food industries. In these industries, internal grain feeders are the most concerning pests because of being the first colonizers of stored grain and transmitting harmful micro-organisms, such as fungi and bacteria, which affect both food quality and human health. The immature stages of these cosmopolitan pests develop and feed inside the grain kernels, easily evading visual analysis in food industries. To avoid the consequent underestimation of contamination by internal pest species, a multiplex PCR approach for the detection and identification of the five most concerning primary pests that develop and feed hidden inside the grain kernels (Rhyzopertha dominica, Sitophilus granarius, S. oryzae, S. zeamais and Sitotroga cerealella) has been developed. Results have demonstrated that the designed protocol can be used for the diagnosis of grain contamination with high sensitivity (0.1 pupa/kilo of rice, except for R. dominica 10 pupae/kilo). This tool proved to be specific when 46 other species potentially present in grain commodities were tested, and to detect all developmental stages of S. zeamais in different kinds of grain (barley, maize, oat, spelt, rice and wheat) and pasta (macaroni). Detection was even possible when grain was treated with CO2. Finally, in order to confirm its applicability in food industries, this method has also been tested in real commercial grain samples from a pasta mill. The multiplex PCR method presented here could be of great help when making commercial decisions aimed at satisfying the current market demands. info:eu-repo/semantics/acceptedVersion |
| Databáze: | OpenAIRE |
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