Electron microscopic studies on human basophils from atopic and nonatopic subjects, using horse radish peroxidase labelled anti-IgE
| Autor: | Ph.C. Brühl, P.J. Stallman, E.H. van Elven |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Hypersensitivity
Immediate Horse radish peroxidase biology Quantitative immunofluorescence Chemistry Immunology Cell Membrane chemical and pharmacologic phenomena hemic and immune systems General Medicine Immunoglobulin E Antibodies Anti-Idiotypic Basophils Immunoenzyme Techniques Peroxidases parasitic diseases biology.protein Immunology and Allergy Humans Electron microscopic Horseradish Peroxidase |
| Zdroj: | International archives of allergy and applied immunology. 54(6) |
| ISSN: | 0020-5915 |
| Popis: | In previous studies, quantitative differences in cell-bound IgE on the basophils from various donors were measured by means of a quantitative immunofluorescence technique. In this study, cell-bound IgE was demonstrated on basophilic granulocytes by immunoelectron microscopy, using horse radish peroxidase labelled anti-IgE. The distribution of IgE on the basophils was studied in both atopic and nonatopic subjects. The intensity of the peroxidase staining on the basophils varied and appeared to correlate with the amount of cell-bound IgE, as estimated by quantitative immunofluorescence. |
| Databáze: | OpenAIRE |
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