Cytotoxicity from cultured cells: Analysis of precursors involved in generation of human cells mediating natural and antibody-dependent cell-mediated cytotoxicity
| Autor: | P. D. Kind, R.B. Herberman, R.P. MacDermott, G.D. Bonnard, J.R. Ortaldo |
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| Rok vydání: | 1979 |
| Předmět: |
Cytotoxicity
Immunologic Rosette Formation Time Factors T-Lymphocytes Immunology Population Receptors Antigen B-Cell chemical and pharmacologic phenomena Cell Communication Cell Separation Receptors Fc Biology Monocytes Mitomycins Interleukin 21 Null cell Humans Cytotoxic T cell education Cytotoxicity Cells Cultured Antilymphocyte Serum Antibody-dependent cell-mediated cytotoxicity education.field_of_study Lymphokine-activated killer cell X-Rays Antibody-Dependent Cell Cytotoxicity Molecular biology Cell biology Killer Cells Natural Interleukin 12 |
| Zdroj: | Cellular Immunology. 48:356-368 |
| ISSN: | 0008-8749 |
| Popis: | Natural cell-mediated cytotoxicity of human peripheral blood lymphocytes natural killer (NK) against K-562 and antibody-dependent cellular cytotoxicity (ADCC) against Chang cells, as measured in a 4-hr 51 Cr release assay, were both completely removed by depletion of Fc receptor-positive (FcR+) cells. After in vitro culture for 7 days, however, NK- and ADCC-like activities spontaneously regenerated. The nature of precursor cells was studied by examination of lymphocyte subpopulations required for generation of this cytotoxicity. After depletion of FcR+ cells from PBL, the following subpopulations were prepared: sheep erythrocyte rosette-forming cells (E+), surface membrane immunoglobulin-positive cells (SmIg+), and null cells (lacking E+, SmIg+, or FcR+ markers). Separate cell types or mixtures were cultured in vitro in medium containing 10% fetal calf serum for 7 days and then tested for NK and ADCC. Whereas unseparated FcR-depleted cells developed substantial cytotoxic activity, each of the subpopulations cultured alone was negative or had low activity. Addition of SmIg+ cells to other cell types had no effect; however, mixture of 80% E+ and 20% null cells resulted in optimal NK and ADCC. It is not presently clear which population the precursors were in. However, the requirement for proliferation by the null cell population but not by the E+ cells (as indicated by sensitivity to radiation and mitomycin C) suggested that the precursors for NK cells may be null cells. |
| Databáze: | OpenAIRE |
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