Genetic characterization of Orobanche cumana populations from the thrace region of Turkey using microsatellite markers
| Autor: | Ahmet Kubilay Barut, Behiye Banu Bilgen, Sefer Demirbaş |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Veterinary medicine Turkey Science Broomrape Locus (genetics) Plant Science genetic analysis Biology 01 natural sciences Molecular marker analysis 030308 mycology & parasitology 03 medical and health sciences Simple sequence repeats Orobanchaceae Botany Genetic variation molecular analysis parasitic plant 0303 health sciences Genetic diversity Fen Dendrogram UPGMA food and beverages population genetics Helianthus annuus Broomrape Helianthus annuus molecular marker analysis simple sequence repeats biology.organism_classification Orobanche Thrace breeding Genetic structure genetic variation Microsatellite biomarker Helianthus Orobanche cernua var. cumana pollution effect 010606 plant biology & botany |
| Zdroj: | Volume: 43, Issue: 1 38-47 Turkish Journal of Botany |
| ISSN: | 1300-008X 1303-6106 |
| Popis: | Orobanche cumana is a parasitic plant that can lead to advanced losses in yield of agricultural lands cultivating sunflower, depending on the sunflower varieties and the level of contamination. In our study, genetic diversity of six O. cumana populations from the Thrace region of Turkey was determined with the help of eight SSR (microsatellite) loci. All SSR loci were polymorphic. A total of 23 alleles were determined for the analyzed 120 samples. Allele number of each SSR locus ranged from two to six. Mean number of alleles (Na = 2.271), effective allele number (Ne = 1.667), Shannon?s information index (I = 0.547), and heterozygosity levels (Ho = 0.207 and He = 0.340) were calculated. A high proportion of the genetic variation (66%) was due to within-population variation and 34% of the diversity was due to among-population variations. Based on the UPGMA dendrogram and STRUCTURE analysis, there were two main clusters. Cluster I was classified into three groups containing four populations from the Kırklareli and Edirne regions. Two populations from Tekirdağ were in cluster II. The information obtained from this study is valuable to provide a significant contribution to studies on the genetic structure, diversity, and race evolution of O. cumana and to the developed crop breeding studies and management strategies for controlling O. cumana infestation. |
| Databáze: | OpenAIRE |
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