HPLC coupled on-line to ESI-MS and a DPPH-based assay for the rapid identification of anti-oxidants in Butea superba
| Autor: | Hubertus Irth, Wilfried M. A. Niessen, C. F. de Jong, Kornkanok Ingkaninan, Ben Bruyneel, Nitra Nuengchamnong |
|---|---|
| Přispěvatelé: | BioAnalytical Chemistry |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization DPPH Electrospray ionization Plant Science Biochemistry High-performance liquid chromatography Antioxidants Analytical Chemistry chemistry.chemical_compound Butea Picrates Drug Discovery Procyanidin B5 Procyanidin B2 Chromatography High Pressure Liquid Chromatography biology Chemistry Biphenyl Compounds Butea superba General Medicine biology.organism_classification Biphenyl compound Hydrazines Complementary and alternative medicine Molecular Medicine SDG 6 - Clean Water and Sanitation Food Science |
| Zdroj: | Nuengchamnong, N, de Jong, C F, Bruyneel, B, Niessen, W M A, Irth, H & Ingkaninan, K 2005, ' HPLC coupled to ESI-MS and a DPPH-based assay for the rapid identification of anti-oxidants in Butea superba ', Phytochemical Analysis, vol. 16, no. 6, pp. 422-8 . https://doi.org/10.1002/pca.865 Phytochemical Analysis, 16(6), 422-8. John Wiley and Sons Ltd |
| ISSN: | 0958-0344 |
| DOI: | 10.1002/pca.865 |
| Popis: | A reversed-phase HPLC coupled on-line to a radical scavenging detection system and MS/MS was developed in order to combine separation, activity determination and structural identification of anti-oxidants in complex mixtures in one run. The sample was separated by HPLC and the eluate split into two flows. The major portion was fed into an electrospray ionisation MS/MS system, while the minor part was mixed with a free radical, 2,2′-diphenyl-1-picrylhydrazyl (DPPH), and the reaction determined spectrophotometrically. The negative peaks, which indicated the presence of anti-oxidant activity, were monitored by measuring the decrease in absorbance at 517 nm. The developed method was successfully applied to the identification of anti-oxidant compounds in a fraction, obtained by solid-phase extraction, of an extract of a Thai medicinal plant, Butea superba Roxb. The anti-oxidant compounds were separated and identified as procyanidin B2, (-)-epicatechin and procyanidin B5. Copyright © 2005 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text