The asymmetric distribution of phosphatidylcholine in rat brain synaptic plasma membranes
| Autor: | Richard C. Crain, Ronit Shina, Eleonora Condrea, Philip Rosenberg |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Cell Membrane Permeability Osmotic shock Kinetics Synaptic Membranes Buffers Biology Secretoglobins Androgen-Binding Protein Article Rats Sprague-Dawley Synapse Cellular and Molecular Neuroscience chemistry.chemical_compound Phospholipid transfer protein Phosphatidylcholine Animals Uteroglobin Tissue Distribution Phospholipid Transfer Proteins Incubation Brain Chemistry Prostatein Cell Biology Saponins Rats Membrane Biochemistry chemistry Cytoplasm Phosphatidylcholines Biophysics Carrier Proteins Synaptosomes |
| Zdroj: | Neurochemistry International |
| ISSN: | 0197-0186 |
| DOI: | 10.1016/0197-0186(93)90012-t |
| Popis: | The distribution of phosphatidylcholine between inner and outer monolayers of rat brain synaptic plasma membrane was investigated by means of a phosphatidylcholine specific exchange protein. About 70% of the total membranal phosphatidylcholine was in the outer leaflet, 33% of which was exposed and readily exchanged in intact synaptosomes while the remainder was exchangeable following osmotic shock. Permeabilization of the synaptic plasma membranes by overnight incubation in buffer or by saponin (< 0.08%) exposed an additional 30% of phosphatidylcholine to exchange, presumably from the inner cytoplasmic leaflet. Phosphatidylcholine is therefore asymmetrically distributed in the synaptosomal plasma membrane, as it is in other plasma membranes. |
| Databáze: | OpenAIRE |
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