Paralytic shellfish poisons produced by the freshwater cyanobacterium Aphanizomenon flos-aquae NH-5
| Autor: | Wayne W. Carmichael, Nik A. Mahmood |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Male
Cyanobacteria Microcystins Bacterial Toxins Ultrafiltration Aphanizomenon flos-aquae (dietary supplement) Toxicology Aphanizomenon High-performance liquid chromatography Mice chemistry.chemical_compound Animals Chromatography High Pressure Liquid Shellfish Toxins Biological Saxitoxin Mice Inbred ICR Chromatography Cyanobacteria Toxins biology Extraction (chemistry) Chromatography Ion Exchange biology.organism_classification chemistry Chromatography Gel Marine Toxins Chromatography Thin Layer |
| Zdroj: | Toxicon. 24:175-186 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/0041-0101(86)90120-0 |
| Popis: | A single filament clonal isolate of Aphanizomenon flos-aquae was made from a water bloom sample taken at a small pond near Durham, New Hampshire, in 1980. When batch cultured the strain was toxic to mice and had an i.p. LD50 of about 5.0 mg/kg. Using an extraction procedure originally designed for paralytic shellfish poisons and other neurotoxins of freshwater cyanobacteria, a purification method was developed. The procedure involved acidified water/ethanol extraction of the cells followed by ultrafiltration, gel filtration, use of C18 cartridges to remove pigments, ion-exchange and high performance liquid chromatography using u.v. detection at 220 or 240 nm. Thin-layer chromatography and high performance liquid chromatography results indicate that Aphanizomenon flos-aquae NH-5 may produce paralytic shellfish poisons, mainly neo-saxitoxin and saxitoxin. Three labile toxins were also detected which were not similar to any of the known paralytic shellfish poisons. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|