SSR-Based Analysis of Genetic Diversity and Structure of Sweet Cherry (Prunus avium L.) from 19 Countries in Europe

Autor: Zsuzsanna Békefi, Gunars Lacis, Gordana Đurić, Andreas Spornberger, Hedi Kaldmäe, Daina Feldmane, Daniela Giovannini, Ossama Kodad, Martin Galik, Ana Cristina Agulheiro-Santos, Marine Blouin-Delmas, Hilde Nybom, Kersti Kahu, Teresa Barreneche, Pavlina Drogoudi, Sanda Stanivuković, Jiri Sedlak, Aleš Vokurka, Felicidad Fernández Fernández, Elisabeth Schüller, Marc Lateur, Mekjell Meland, Sorina Sirbu, Matthew Ordidge, Monika Höfer, María Cárcamo de la Concepción, José Quero-Garcia
Přispěvatelé: Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Greenwich, Unité Expérimentale Arboricole (UE ARBO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Reading (UOR), Swedish University of Agricultural Sciences (SLU), Institute of Horticulture, Research and Breeding Institute of Pomology Holovousy (VSUO), Norsk institutt for bioøkonomi=Norwegian Institute of Bioeconomy Research (NIBIO), Institute of Agricultural and Environmental Sciences (IAES), Estonian University of Life Sciences (EMU), National Agricultural Research and Innovation Center (NARIC), University of Banja Luka, Julius Kühn-Institute, National Agriculture and Food Centre – Research Institute of Plant Production, Universität für Bodenkultur Wien = University of Natural Resources and Life [Vienne, Autriche] (BOKU), Research Station for Viticulture and Enology Murfatlar, Calea Bucuresti, nr. 2, Murfatlar, Romania, Institute of Plant Breeding and Genetic Resources, Universidade de Évora, Ecole Nationale d'Agriculture de Meknès (ENA), University of Zagreb, Centre Wallon de Recherches Agronomiques (CRA-W), NIAB EMR, Partenaires INRAE, CREA, Research Centre for Olive, This research was funded by the European Cooperative Program for Plant Genetic Resources Networks (ECP GR): EU.CHERRY project (ECPGR Activity Grant Scheme – Second Call, 2015). The COST Action Cherry funded part of accession sampling and the first meeting of EU.CHERRY project held in Naoussa, Greece, in April 2016. PD was financed by the European Union and Greek national funds through the Operational Program Competitiveness, Entrepreneurship, and Innovation, under the call RESEARCH–CREATE-INNOVATE (project code: Τ1EDK-05438)., European Project, Unité d'arboriculture (BORDX ARBORI UE), Norwegian Institute of Bioeconomy Research (NIBIO), University of Natural Resources and Life Sciences (BOKU)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Plants, Vol 10, Iss 1983, p 1983 (2021)
Plants
Plants, 2021, 10, pp.1983. ⟨10.3390/plants10101983⟩
Volume 10
Issue 10
Plants, MDPI, 2021, 10, pp.1983. ⟨10.3390/plants10101983⟩
ISSN: 2223-7747
DOI: 10.3390/plants10101983⟩
Popis: Sweet cherry (Prunus avium L.) is a temperate fruit species whose production might be highly impacted by climate change in the near future. Diversity of plant material could be an option to mitigate these climate risks by enabling producers to have new cultivars well adapted to new environmental conditions. In this study, subsets of sweet cherry collections of 19 European countries were genotyped using 14 SSR. The objectives of this study were (i) to assess genetic diversity parameters, (ii) to estimate the levels of population structure, and (iii) to identify germplasm redundancies. A total of 314 accessions, including landraces, early selections, and modern cultivars, were monitored, and 220 unique SSR genotypes were identified. All 14 loci were confirmed to be polymorphic, and a total of 137 alleles were detected with a mean of 9.8 alleles per locus. The average number of alleles (N = 9.8), PIC value (0.658), observed heterozygosity (Ho = 0.71), and expected heterozygosity (He = 0.70) were higher in this study compared to values reported so far. Four ancestral populations were detected using STRUCTURE software and confirmed by Principal Coordinate Analysis (PCoA), and two of them (K1 and K4) could be attributed to the geographical origin of the accessions. A N-J tree grouped the 220 sweet cherry accessions within three main clusters and six subgroups. Accessions belonging to the four STRUCTURE populations roughly clustered together. Clustering confirmed known genealogical data for several accessions. The large genetic diversity of the collection was demonstrated, in particular within the landrace pool, justifying the efforts made over decades for their conservation. New sources of diversity will allow producers to face challenges, such as climate change and the need to develop more sustainable production systems.
Databáze: OpenAIRE
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