The relationship between apoptosis, chromatin configuration, histone modification and competence of oocytes: A study using the mouse ovary-holding stress model
| Autor: | Fei Chen, Liu-Zhu Pan, Jing-He Tan, Jie Zhang, You-Wei Li, Jiang Zhu, Juan Lin, Ming-Ju Sun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Fas Ligand Protein Apoptosis Oogenesis Fas ligand Article Histones 03 medical and health sciences Mice 0302 clinical medicine Ovarian Follicle Protein Domains Heterochromatin medicine Histone code Animals Epigenetics 030219 obstetrics & reproductive medicine Multidisciplinary Cumulus Cells Granulosa Cells biology Ovary Temperature Acetylation Oocyte Molecular biology Chromatin Lymphoproliferative Disorders Recombinant Proteins Cell biology Histone Code Mice Inbred C57BL 030104 developmental biology Histone medicine.anatomical_structure biology.protein Oocytes Female Protein Processing Post-Translational Cell Nucleolus Signal Transduction |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The epigenetic factors causing competence differences between SN (surrounded nucleolus) and NSN (non-surrounded nucleolus) oocytes, the significance for the increased histone acetylation and methylation in SN oocytes, and whether chromatin configuration or histone modification determines oocyte competence, are unclear. This study has addressed these issues by using the ovary-holding (OH) stress models where oocyte SN configuration was uncoupled from histone modifications and developmental potential. Prepubertal mouse ovaries containing high percentages of NSN oocytes were preserved at 37 or 39 °C for 1 or 2 h before examination for oocyte chromatin configuration, developmental competence, histone modification and apoptosis. Whereas 1-h OH at 37 °C caused a moderate apoptosis with increased oocyte competence, improved histone modification and a normal NSN-to-SN transition, harsher OH conditions induced a severe apoptosis with decreased oocyte competence, impaired histone modification and a pseudo (premature) NSN-to-SN transition. Observations on Fas/FasL expression and using the gld (generalized lymphoproliferative disorder) mice harboring FasL mutations indicated that OH triggered oocyte apoptosis with activation of the Fas signaling. It was concluded that OH stress caused oocyte apoptosis with activation of the Fas/FasL system and that oocyte competence was more closely correlated with histone modification than with chromatin configuration. |
| Databáze: | OpenAIRE |
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