The Effects of Intraperitoneal Pentoxifylline Treatment in Rat Pups With Hypoxic-Ischemic Encephalopathy
| Autor: | Mustafa Akcakus, Gönül Tezel, Hakan Aldemir, Emel Sahin, Sadi Köksoy, Osman Öztekin, Salih Kalay, Nihal Oygür |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Interleukin-1beta Encephalopathy Ischemia Brain damage Statistics Nonparametric Hypoxic Ischemic Encephalopathy Pentoxifylline Developmental Neuroscience Internal medicine medicine Animals RNA Messenger Rats Wistar Caspase 3 Tumor Necrosis Factor-alpha business.industry Brain Hypoxia (medical) medicine.disease Rats Disease Models Animal Endocrinology Animals Newborn Gene Expression Regulation Neurology Anesthesia Hypoxia-Ischemia Brain Pediatrics Perinatology and Child Health Systemic administration Female Tumor necrosis factor alpha Neurology (clinical) medicine.symptom business Injections Intraperitoneal Platelet Aggregation Inhibitors medicine.drug |
| Zdroj: | Pediatric Neurology. 49:319-323 |
| ISSN: | 0887-8994 |
| DOI: | 10.1016/j.pediatrneurol.2013.05.011 |
| Popis: | Background The aim of this study was to evaluate the effects of postischemic treatment with pentoxifylline on the cytokine gene expressions and neuronal apoptosis in neonatal rat model of hypoxic-ischemic encephalopathy. Methods Seven-day-old Wistar rat pups (n = 40) of either sex, delivered spontaneously, were used in this experimental study. Control group (n = 8): after median neck incision was made, neither ligation nor hypoxia was performed, ischemia group (n = 16): 0.5 mL of saline was injected intraperitoneally immediately after hypoxia. Pentoxifylline and ischemia groups (n = 16): the rat pups were administered intraperitoneally 60 mg/kg of pentoxifylline immediately after hypoxia. Eight rats from ischemia and pentoxifylline + ischemia groups were sacrificed 4 and 24 hours after drug administration. Control group mice were decapitated 4 hours after hypoxia. Caspase-3 activity, interleukin-1β, and tumor necrosis factor-α messenger RNA expression levels were studied in the left half of the brain. Results Induction of cerebral ischemia increased tumor necrosis factor-α and interleukin-1β messenger RNA expression levels significantly at 4 hours and 24 hours following ischemia in the left ischemic hemispheres in the ischemia group as compared with the control group. Systemic administration of pentoxifylline immediately after hypoxic-ischemic encephalopathy significantly reduced the tumor necrosis factor-α and interleukin-1β messenger RNA expression levels in ischemic tissue as compared with the ischemia group. Caspase-3 activities in the left half of the brains of ischemia group were found to be increased significantly as compared with control group. Caspase-3 activities in the brains of pentoxifylline + ischemia groups were significantly lower than in that of ischemia group. Conclusions Based on the significantly lower interleukin-1β and tumor necrosis factor-α gene expression measured after 4 and 24 hours and significantly reduced caspase-3 activity measured colorimetrically in the animals treated with pentoxifylline, our findings suggest that pentoxifylline may reduce brain damage due to hypoxic-ischemic injury. |
| Databáze: | OpenAIRE |
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