Chitosan immobilized novel peroxidase from Azadirachta indica: Characterization and application
| Autor: | Jyoti Rani, Swati Singh, Ajit Kumar Shasany, Veda P. Pandey, Manika Awasthi, Sameeksha Tiwari, Nivedita Jaiswal, Upendra N. Dwivedi |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Color 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound Structural Biology 010608 biotechnology Metals Heavy parasitic diseases Enzyme Stability Acetone Coloring Agents Molecular Biology Thermostability Peroxidase Chitosan Chromatography Azadirachta biology Chemistry Butanol Temperature Substrate (chemistry) General Medicine Hydrogen-Ion Concentration biology.organism_classification Enzymes Immobilized Molecular Weight Kinetics 030104 developmental biology Pyrogallol biology.protein Solvents Salts Guaiacol |
| Zdroj: | International journal of biological macromolecules. 104 |
| ISSN: | 1879-0003 |
| Popis: | In the present paper, a peroxidase was purified from the leaves of a medicinal tree, namely Azadirachta indica, to 45.2 folds with overall recovery of 61%. Based on the subunit size, the purified peroxidase was suggested to be a monomeric structure of size 50kDa and exhibited good thermostability as it was fully stable at 65°C for 1hr and also retained about 73% activity at 70°C till 30min. The substrate affinity was found to be in order of guaiacol>pyrogallol>o-dianisidine. The purified peroxidase was found to be insensitive towards high concentrations of Na+, Ca2+, Mg2+ and Mn2+. Heavy metals, namely Cs2+, Co2+ and Cd2+ activated the peroxidase while that of Hg2+ deactivated the peroxidase in concentration dependent manner. The purified peroxidase exhibited tolerance towards organic solvents in order of ethanol>butanol>isopropanol>acetone. Immobilization of purified peroxidase by entrapment into chitosan beads led to shift in its optimum pH from pH 5 to 7 and considerable enhancement in dye decolorization ability as compared to that of free enzyme. Thus, based on all the above properties, it may be suggested that the purified A. indica peroxidase is a promising candidate for industrial applications. |
| Databáze: | OpenAIRE |
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