Oxygen Availability during Growth Modulates the Phytochemical Profile and the Chemo-Protective Properties of Spinach Juice

Autor: Luca Forti, Laura Arru, Annamaria Buschini, Francesco Milano, Silvia Fornaciari, Francesca Mussi, Meltem Altunoz
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Spinacia
Antioxidant
HT29 cell line
Cell Survival
medicine.medical_treatment
Liquid Chromatography-Mass Spectrometry
Spinacia oleracea L
lcsh:QR1-502
antioxidant activity
Anti-proliferative activity
Antioxidant activity
Comet Assay
medicine.disease_cause
01 natural sciences
Biochemistry
lcsh:Microbiology
Antioxidants
Mass Spectrometry
Article
03 medical and health sciences
Spinacia oleracea
medicine
Humans
Food science
Molecular Biology
Cell Proliferation
biology
Abiotic stress
Chemistry
Chemistry
Physical
Plant Extracts
food and beverages
Biological activity
biology.organism_classification
Antineoplastic Agents
Phytogenic
Comet assay
Fruit and Vegetable Juices
Oxygen
030104 developmental biology
Phytochemical
Spinach
anti-proliferative activity
Drug Screening Assays
Antitumor
HT29 Cells
Oxidative stress
010606 plant biology & botany
Chromatography
Liquid
Zdroj: Biomolecules
Biomolecules, Vol 9, Iss 2, p 53 (2019)
Volume 9
Issue 2
ISSN: 2218-273X
Popis: Fruits and vegetables are a good source of potentially biologically active compounds. Their regular consumption in the human diet can help reduce the risk of developing chronic diseases such as cardiovascular diseases and cancer. Plants produce additional chemical substances when subject to abiotic stress or infected by microorganisms. The phytochemical profile of spinach leaves (Spinacia oleracea L.), which is a vegetable with widely recognized health-promoting activity, has been affected by applying root hypoxic and re-oxygenation stress during plant growth. Leaf juice at different sampling times has been subject to liquid chromatography mass spectrometry (LC-MSn) analysis and tested on the human colorectal adenocarcinoma cell line HT29 by using the Comet assay. The cells were previously treated with H2O2 to simulate the presence of an oxidative stress (as in colon cancer condition) and the leaf juice application resulted in a significant antioxidant and protective in vitro effect. The duration of the hypoxic/re-oxygenation stress imposed on the plant reflects the antioxidant leaf juice content. After hypoxic stress (24 h) and reoxygenation (2 h), we show a decrease (50%) of the relative abundance of the principal identified antioxidant molecules but a higher antioxidant activity of the spinach juice on HT29 cells (20%). Data shows a complex relation between plant growing conditions and the modulation of secondary metabolites content in leaf juice that results in different chemo-protective activities in colon cancer cells.
Databáze: OpenAIRE
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