Benznidazole affects expression of Th1, Th17 and Treg cytokines during acute experimental Trypanosoma cruzi infection

Autor: Eliana Peresi Lordelo, João Pessoa Araújo Júnior, Fernanda De Nuzzi Dias, Larissa Ragozo Cardoso de Oliveira, Carlos Roberto Gonçalves de Lima, Mariana Gatto, Cilmery Suemi Kurokawa, Rodrigo Mattos dos Santos
Přispěvatelé: Universidade Estadual Paulista (Unesp), University of Western São Paulo (Unoeste)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: The Journal of Venomous Animals and Toxins Including Tropical Diseases
Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 23, Article number: 47, Published: 01 FEB 2018
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 23, Iss 1, Pp 1-9 (2017)
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Journal of Venomous Animals and Toxins including Tropical Diseases v.23 2017
The Journal of venomous animals and toxins including tropical diseases
ISSN: 1678-9199
Popis: Made available in DSpace on 2018-12-11T17:16:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-12-12. Added 1 bitstream(s) on 2021-07-15T15:21:57Z : No. of bitstreams: 1 S1678-91992017000100320.pdf: 615232 bytes, checksum: 21546f283e7bcd222c4e98d85ca7e698 (MD5) Background: The present study evaluated the effect of treatment with benznidazole on mRNA expression of IFN-γ, IL-17, IL-10, TGF-β and FoxP3 in spleen and heart tissue of BALB/c mice in the acute phase of an experimental infection with Trypanosoma cruzi, strains JLP or Y. Methods: The mRNA expression of cytokines and parasite load were assessed by q-PCR. Dependent groups were compared using Student's paired t-test and independent groups were compared using Student's unpaired t-test. Results: Infection with the JLP or Y strains increased expression of IFN-γ in the heart and of IL-10 and IL-17 in the spleen and heart compared to uninfected animals. Treatment increased the expression of IFN-γ and decreased the expression of IL-17, IL-10, TGF- β and Foxp3 in spleen and heart tissue compared to untreated infected animals. Conclusion: Benznidazole can induce Th1 profile in the initial of the acute phase. The treatment decreased the parasite load in both organs, although the number of parasites in Y-strain-infected mice remained high. The data suggest that benznidazole may modulate cytokine expression in infection and can be dependent of the strain. However, treatment was not fully effective in the infection provoked by Y strain, probably due to the characteristics of the strain itself. Department of Tropical Diseases Botucatu Medical School São Paulo State University (UNESP - Univ Estadual Paulista), Av. Professor Mario Rubens Guimaraes Montenegro, s/n Department of Microbiology and Immunology Botucatu Biosciences Institute São Paulo State University (UNESP - Univ Estadual Paulista) Department of Biological Sciences School of Pharmaceutical Sciences São Paulo State University (UNESP - Univ Estadual Paulista) Department of Immunology University of Western São Paulo (Unoeste) Department of Tropical Diseases Botucatu Medical School São Paulo State University (UNESP - Univ Estadual Paulista), Av. Professor Mario Rubens Guimaraes Montenegro, s/n Department of Microbiology and Immunology Botucatu Biosciences Institute São Paulo State University (UNESP - Univ Estadual Paulista) Department of Biological Sciences School of Pharmaceutical Sciences São Paulo State University (UNESP - Univ Estadual Paulista)
Databáze: OpenAIRE
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