Camptothecin induces the transit of FasL trimers to the cell surface in apoptotic HEp-2 cells
| Autor: | Sergio Hugo Sánchez-Rodríguez, Esperanza Avalos-Díaz, Rafael Herrera-Esparza, Erendira Lopez-Robles, Roxana Ramírez-Sandoval, Juan-Jose Bollain-y-Goytia, Esteban Meza-Lamas |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Fas Ligand Protein
Transcription Genetic Cell chemical and pharmacologic phenomena Apoptosis DNA Fragmentation Biology Biochemistry Fas ligand Article Amino Acid Chloromethyl Ketones Paracrine signalling Cell Line Tumor medicine In Situ Nick-End Labeling Humans fas Receptor Autocrine signalling Poly-ADP-Ribose Binding Proteins Protein Structure Quaternary Molecular Biology Deoxyribonucleases Caspase 3 hemic and immune systems Cell Biology Fas receptor Molecular biology Cell biology Protein Transport medicine.anatomical_structure Cytoplasm Tumor necrosis factor alpha Camptothecin biological phenomena cell phenomena and immunity Subcellular Fractions |
| Zdroj: | Cellularmolecular biology letters. 11(3) |
| ISSN: | 1689-1392 |
| Popis: | Fas ligand (L) is a membrane protein from the tumor necrosis factor (TNF) family. It induces apoptosis upon contact with its Fas/CD95/APO1 receptor. Trimerization of FasL on the surface of effector cells is essential in the binding of the Fas trimer of the target cells. The receptor then recruits an adaptor and caspase-like proteins which lead apoptosis. This paper reports on the fate of FasL in HEp-2 cells committed to apoptosis by induction with campthotecin. Our main results demonstrated that in non-apoptotic cells, FasL aggregates in the cytoplasm forming trimers of 120 kDa. Apoptosis increases the trimeric FasL species, but also induces its dissociation into monomers of 35 kDa. In conclusion, camptothecin appears to perturb the Fas and FasL segregation in the cytoplasm by promoting the transit of FasL to the cell surface, thus fostering a process of autocrine or paracrine apoptosis. FasL is trimerized prior to Fas/FasL complex formation, and after apoptosis, FasL undergoes an intense turnover. |
| Databáze: | OpenAIRE |
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