Development and evaluation of doxorubicin self nanoemulsifying drug delivery system with Nigella Sativa oil against human hepatocellular carcinoma
| Autor: | Asif Jafri, Afreen Usmani, Anuradha Mishra, Arshad |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Carcinoma
Hepatocellular Cell Survival Nigella sativa Intracellular Space Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) 02 engineering and technology Pharmacology Self nanoemulsifying 03 medical and health sciences 0302 clinical medicine medicine Humans Plant Oils Doxorubicin Viability assay Cell Nucleus Viscosity business.industry Liver Neoplasms Water food and beverages Drug Synergism Hep G2 Cells General Medicine 021001 nanoscience & nanotechnology medicine.disease Nanostructures 030220 oncology & carcinogenesis Hepatocellular carcinoma Hepg2 cells Nigella sativa oil Drug delivery Thermodynamics Emulsions Reactive Oxygen Species 0210 nano-technology business Biotechnology medicine.drug |
| Zdroj: | Artificial Cells, Nanomedicine, and Biotechnology. 47:933-944 |
| ISSN: | 2169-141X 2169-1401 |
| Popis: | The development of self nano emulsifying co-delivery system of doxorubicin and Nigella sativa oil for potentiating the anticancer effects against HepG2 cell lines.SNEDDS were formulated by using Labrafil and N. sativa oil (3:2% w/w), Kolliphor RH40 (15% w/w), glycerol (5% w/w) as oil phase, surfactant and co-surfactant while deionized water (75% v/v) used as an aqueous phase. Optimized SNEDDS was evaluated for drug release and in vitro anticancer efficacy in liver cancer (HepG2) cell line.The selected formulation (F6) has a mean particle size of 79.7 nm with PDI 0.098 and the minimum viscosity of 16.42 cps with % transmittance of 1.332 with maximum drug release of 96.968% in 32 h as compared to DOX alone. Stability data showed stable emulsion in both 25The overall study displayed that co-delivery of DOX and Nigella sativa oil in the form of SNEDDS may be an efficient carrier for further in vivo studies using oral delivery in human hepatocellular carcinoma in mammals. |
| Databáze: | OpenAIRE |
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