Biocompatibility and functionality of a tissue-engineered living corneal stroma transplanted in the feline eye
| Autor: | Fatma Zaguia, Marie Claude Perron, Xinling Liu, Isabelle Brunette, Marie Boulze Pankert, Benjamin Goyer, Myriam Bareille, J. Douglas Cameron, Stéphanie Proulx |
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| Přispěvatelé: | Université de Montréal. Faculté de médecine. Département d'ophtalmologie |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Stromal cell genetic structures medicine.medical_treatment Corneal Stroma Transplantation Heterologous law.invention Corneal Transplantation Cellular and Molecular Neuroscience Tissue engineering Confocal microscopy law Cornea Medicine Animals Humans Corneal transplantation Cells Cultured Microscopy Confocal Tissue Engineering business.industry Histocompatibility Testing Endothelium Corneal Graft Survival Eye bank Histology eye diseases Sensory Systems Reinnervation Transplantation Ophthalmology Disease Models Animal medicine.anatomical_structure Corneal substitute Cats Cell culture sense organs business Tomography Optical Coherence |
| Zdroj: | Investigative ophthalmologyvisual science. 55(10) |
| ISSN: | 1552-5783 |
| Popis: | Purpose Corneal tissue shortage has become a major concern worldwide, which has motivated the search for alternative solutions to eye bank human eyes for corneal transplantation. Minimally invasive lamellar transplantation and tissue engineering may offer new opportunities for the rehabilitation of diseased corneas. The aim of this study was to evaluate the biocompatibility and functionality of stromal lamellar grafts tissue-engineered (TE) in vitro and transplanted in vivo in the cornea of a feline model. Methods The corneal stromas were engineered in culture from corneal stromal cells using the self-assembly approach, without the addition of exogenous material or scaffold. Eight healthy animals underwent two intrastromal grafts in one eye and the contralateral eye was used as a control. Animals were followed with slit-lamp ophthalmic examination, corneal esthesiometry and optical coherent tomography. Confocal microscopy, immunofluorescence, histology, and transmission electron microscopy (TEM) were performed at 4 months. Results Four months after transplantation, the TE-stromal grafts were transparent, functional, and well tolerated by the eye. All grafts remained avascular, with no signs of immune rejection, despite a short course of low-dose topical steroids. Corneal sensitivity returned to preoperative level and reinnervation of the grafts was confirmed by confocal microscopy and immunofluorescence. Histology and TEM of the TE-grafts showed a lamellar stromal structure with regular collagen fibril arrangement. Conclusions These results open the way to an entirely new therapeutic modality. Intracorneal filling using a biocompatible, transparent, and malleable TE-stroma could be the basis for multiple types of novel therapeutic options in corneal interventional surgery. |
| Databáze: | OpenAIRE |
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