Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice
| Autor: | Louis J. Picker, Jay A. Nelson, Patrizia Caposio, Craig N. Kreklywich, Daniel N. Streblow, Lindsey B. Crawford, Patricia P. Smith, Rebecca Tempel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male 0301 basic medicine Human cytomegalovirus Science viruses Transplantation Heterologous Cytomegalovirus Context (language use) Mice SCID Thymus Gland CD8-Positive T-Lymphocytes Biology Antibodies Viral Article Virus Mice 03 medical and health sciences 0302 clinical medicine Immune system Mice Inbred NOD medicine Animals Humans Bone Marrow Transplantation Multidisciplinary Effector virus diseases biochemical phenomena metabolism and nutrition medicine.disease Virology Liver Transplantation Transplantation Disease Models Animal 030104 developmental biology Immunoglobulin M Immunoglobulin G Cytomegalovirus Infections Immunology biology.protein Medicine Female Antibody Immunologic Memory CD8 030215 immunology |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-01051-5 |
| Popis: | The strict species specificity of Human Cytomegalovirus (HCMV) has impeded our understanding of antiviral adaptive immune responses in the context of a human immune system. We have previously shown that HCMV infection of human hematopoietic progenitor cells engrafted in immune deficient mice (huNSG) results in viral latency that can be reactivated following G-CSF treatment. In this study, we characterized the functional human adaptive immune responses in HCMV latently-infected huBLT (humanized Bone marrow-Liver-Thymus) mice. Following infection, huBLT mice generate human effector and central memory CD4+ and CD8+ T-cell responses reactive to peptides corresponding to both IE and pp65 proteins. Additionally, both HCMV specific IgM and IgG B-cell responses with the ability to neutralize virus were detected. These results indicate that the HCMV huBLT mouse model may provide a valuable tool to study viral latency and reactivation as well as evaluate HCMV vaccines and immune responses in the context of a functional human immune system. |
| Databáze: | OpenAIRE |
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