Active liquid crystals powered by force-sensing DNA-motor clusters
| Autor: | Zvonimir Dogic, Michael F. Hagan, Alexandra M. Tayar |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
1.1 Normal biological development and functioning Kinesins FOS: Physical sciences Bioengineering 02 engineering and technology Biosensing Techniques Condensed Matter - Soft Condensed Matter 01 natural sciences Microtubules molecular motors Liquid crystal Underpinning research Tubulin 0103 physical sciences Molecular motor Physics - Biological Physics 010306 general physics Nanoscopic scale Mechanical Phenomena Mesoscopic physics Multidisciplinary Molecular Motor Proteins DNA 021001 nanoscience & nanotechnology Active matter Biomechanical Phenomena Liquid Crystals Chemical physics Biological Physics (physics.bio-ph) Physical Sciences Kinesin Soft Condensed Matter (cond-mat.soft) DNA force sensor 0210 nano-technology active matter Linker Fluorescence anisotropy Protein Binding |
| Zdroj: | Proc Natl Acad Sci U S A Proceedings of the National Academy of Sciences of the United States of America, vol 118, iss 30 |
| DOI: | 10.48550/arxiv.2106.14097 |
| Popis: | Cytoskeletal active nematics exhibit striking non-equilibrium dynamics that are powered by energy-consuming molecular motors. To gain insight into the structure and mechanics of these materials, we design programmable clusters in which kinesin motors are linked by a double-stranded DNA linker. The efficiency by which DNA-based clusters power active nematics depends on both the stepping dynamics of the kinesin motors and the chemical structure of the polymeric linker. Fluorescence anisotropy measurements reveal that the motor clusters, like filamentous microtubules, exhibit local nematic order. The properties of the DNA linker enable the design of force-sensing clusters. When the load across the linker exceeds a critical threshold the clusters fall apart, ceasing to generate active stresses and slowing the system dynamics. Fluorescence readout reveals the fraction of bound clusters that generate interfilament sliding. In turn, this yields the average load experienced by the kinesin motors as they step along the microtubules. DNA-motor clusters provide a foundation for understanding the molecular mechanism by which nanoscale molecular motors collectively generate mesoscopic active stresses, which in turn power macroscale non-equilibrium dynamics of active nematics. Comment: main text: text 19 pages, 6 figures. Supplementary information: text 9 pages, 12 figures |
| Databáze: | OpenAIRE |
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