Utilization of real-time PCR to detect Rangifer Cornu contamination in Cervi Parvum Cornu
| Autor: | Rack Seon Seong, Dong Sup Kim, Seung Yeup Chang, Hyoung Jin Kim, Young Hun Shim, Shin Jung Kang |
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| Rok vydání: | 2011 |
| Předmět: |
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Biological Products Chromatography Deer Organic Chemistry Pharmacology toxicology technology industry and agriculture Antlers DNA macromolecular substances Biology Contamination Polymerase Chain Reaction Sensitivity and Specificity law.invention Real-time polymerase chain reaction law Drug Discovery Animals Molecular Medicine Pcr method Primer (molecular biology) Drug Contamination Polymerase chain reaction DNA Primers |
| Zdroj: | Archives of Pharmacal Research. 34:237-244 |
| ISSN: | 1976-3786 0253-6269 |
| DOI: | 10.1007/s12272-011-0209-x |
| Popis: | Cervi parvum cornu (CPC) is a well-known ethnopharmacological source, whereas Rangifer cornu (RC) is not considered to be a major source. CPC is distributed in sliced form. Addition of RC to CPC has become an issue in CPC distribution because the appearance of sliced RC is not different from sliced CPC. Therefore, a real-time polymerase chain reaction (PCR) method was developed in this study to detect contaminating RC in CPC. The C-VIC and R-FAM primer/probe sets were designed to specifically amplify CPC and RC DNA, respectively. The specificities and sensitivities of real-time PCR using two primer/probe sets and the applicability of the real-time PCR to powder mixtures, which involved mixtures of powdered CPC and powdered RC in diverse ratios, were evaluated. Real-time PCR using C-VIC and R-FAM primer/probe sets specifically and sensitively amplified both CPC and RC DNA. Furthermore, real-time RCR sensitively detected RC DNA in the powder mixtures of CPC and RC. These results indicate that this real-time PCR method using two primer/probe sets is sufficiently applicable for the detection of contaminant RC in CPC. |
| Databáze: | OpenAIRE |
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