Overexpression of BCL-2, BCL-X, and BAX in Primary Central Nervous System Lymphomas that Occur in Immunosuppressed Patients
| Autor: | Sophie Camilleri-Broët, Khé Hoang Xuan, Karima Mokhtari, Martine Raphael, Jean-Jacques Hauw, Antoine Martin, Michel Arborio, Philippe Camparo |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Herpesvirus 4 Human Pathology medicine.medical_specialty Programmed cell death Lymphoma B-Cell Necrosis medicine.medical_treatment bcl-X Protein Apoptosis Biology Virus Pathology and Forensic Medicine Immunoenzyme Techniques Viral Matrix Proteins Immunocompromised Host Immunophenotyping Proto-Oncogene Proteins hemic and lymphatic diseases In Situ Nick-End Labeling medicine Humans In Situ Hybridization Aged bcl-2-Associated X Protein Aged 80 and over Brain Neoplasms Immunosuppression Middle Aged Proto-Oncogene Proteins c-bcl-2 Gliosis RNA Viral Immunohistochemistry Female Lymphoma Large B-Cell Diffuse medicine.symptom |
| Zdroj: | Modern Pathology. 13:158-165 |
| ISSN: | 0893-3952 |
| DOI: | 10.1038/modpathol.3880030 |
| Popis: | In contrast to primary central nervous system lymphomas (PCNSLs) that occur in immunocompetent patients, most of those that occur in immunosuppressed patients are associated with Epstein-Barr virus (EBV). BCL-2–related proteins either block or promote cell death, forming homo- or heterodimers with each other. LMP-1, EBV latent protein, has been shown to upregulate BCL-2 and BCL-XL. This observation suggests that these proteins may be involved in the transformation process of EBV-infected cells. Twenty-three cases of PCNSLs were studied: 12 of the patients were immunosuppressed, and 11 were immunocompetent. For all cases, we collected clinical information, histologic data, and immunophenotype and tested for the presence of EBV (EBER-1, LMP-1). Apoptosis was assessed by the TdT-mediated dUTP-biotin nick-end labeling method and quantified by image analysis. In three cases, electron microscopy was performed. The BCL-2 family proteins (BCL-2, BCL-X, MCL1, and BAX) and p53 expression were studied by immunohistochemistry on paraffin slides. All cases were classified as diffuse large B-cell lymphomas. PCNSLs in immunosuppressed patients were characterized by EBV association, necrosis, important gliosis, and numerous macrophages. There was no significant difference between the two groups regarding the TdT-mediated dUTP-biotin nick-end labeling staining (P =.08). In contrast, PCNSLs in immunosuppressed patients were shown to express high levels of BCL-2, BCL-X, and BAX in more than 80% of tumor cells in 7, 10, and 11 cases, respectively. In immunocompetent patients, only one case showed a high level of BCL-2 expression in more than 80% of the cells, whereas BCL-X and BAX were overexpressed in two cases. These differences are significant (P |
| Databáze: | OpenAIRE |
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