Distinct light-initiated gene expression and cell cycle programs in the shoot apex and cotyledons of Arabidopsis

Autor: Gerrit T.S. Beemster, James A. H. Murray, László Bögre, Sarah M. de Jager, Zoltán Magyar, Safina Khan, Saul Hazeldine, Enrique López-Juez, Edyta Dillon, Hugh P. Shanahan
Jazyk: angličtina
Rok vydání: 2008
Předmět:
ROOT-GROWTH
food.ingredient
Light
Transcription
Genetic
APICAL MERISTEM
Photosynthetic Reaction Center Complex Proteins
Arabidopsis
Gene Expression
Amino Acid Sequence Arabidopsis/cytology/genetics/*radiation effects Cell Cycle/*radiation effects Cotyledon/*cytology Gene Expression/*radiation effects Genes
Plant Science
CIRCADIAN CLOCK
Faculty of Science\Computer Science
Genes
Plant
Polymerase Chain Reaction
food
Cryptochrome
Arabidopsis thaliana
Primordium
Amino Acid Sequence
SEEDLING DE-ETIOLATION
GENOME-WIDE ANALYSIS
Transcription factor
TRANSCRIPTION-FACTOR
Research Articles
Genetics
CHLOROPLAST DEVELOPMENT
biology
COP9 SIGNALOSOME
PLANT DEVELOPMENT
Cell Cycle
fungi
Biology and Life Sciences
food and beverages
Cell Biology
Faculty of Science\Biological Science
Meristem
biology.organism_classification
Apex (geometry)
Cell biology
RECEPTOR FAMILY
Multigene Family
Genetic/radiation effects
Plant *Light Multigene Family Photosynthetic Reaction Center Complex Proteins/physiology Plant Shoots/*cytology Polymerase Chain Reaction Transcription
Cotyledon
Plant Shoots
Zdroj: Plant Cell, 20 (4)
PLANT CELL
ISSN: 1040-4651
DOI: 10.1105/tpc.107.057075
Popis: In darkness, shoot apex growth is repressed, but it becomes rapidly activated by light. We show that phytochromes and cryptochromes play largely redundant roles in this derepression in Arabidopsis thaliana. We examined the light activation of transcriptional changes in a finely resolved time course, comparing the shoot apex (meristem and leaf primordia) and the cotyledon and found >5700 differentially expressed genes. Early events specific to the shoot apices included the repression of genes for Really Interesting New Gene finger proteins and basic domain/leucine zipper and basic helix-loop-helix transcription factors. The downregulation of auxin and ethylene and the upregulation of cytokinin and gibberellin hormonal responses were also characteristic of shoot apices. In the apex, genes involved in ribosome biogenesis and protein translation were rapidly and synchronously induced, simultaneously with cell proliferation genes, preceding visible organ growth. Subsequently, the activation of signaling genes and transcriptional signatures of cell wall expansion, turgor generation, and plastid biogenesis were apparent. Furthermore, light regulates the forms and protein levels of two transcription factors with opposing functions in cell proliferation, E2FB and E2FC, through the Constitutively Photomorphogenic1 (COP1), COP9-Signalosome5, and Deetiolated1 light signaling molecules. These data provide the basis for reconstruction of the regulatory networks for light-regulated meristem, leaf, and cotyledon development.
Databáze: OpenAIRE
Externí odkaz: