Analysis of multiple Leptospira interrogans serovar Canicola vaccine proteomes and identification of LipL32 as a biomarker for potency
| Autor: | P. C. Humphryes, A. Gielbert, N. G. Coldham, G. Thomson, Mark E. Weeks |
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| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Serotype Proteome Lipoproteins Clinical Biochemistry Immunology Leptospira interrogans serovar canicola Biology Mass Spectrometry Microbiology Bacterial Proteins In vivo Leptospira Cricetinae Immunology and Allergy Potency Animals Technology Pharmaceutical Electrophoresis Gel Two-Dimensional Vaccines biology.organism_classification Antibodies Bacterial Bacterial vaccine Vaccine Potency Limulus amebocyte lysate Bacterial Vaccines Female Biomarkers Bacterial Outer Membrane Proteins Chromatography Liquid |
| Zdroj: | Clinical and vaccine immunology : CVI. 19(4) |
| ISSN: | 1556-679X |
| Popis: | The current batch potency test forLeptospira interrogansserovar Canicola vaccines requires the use of a large number of hamsters and has severe effects (i.e., hepatic and renal failure resulting in death); while this vaccine is effective, a safer, cheaper, more ethical replacement is desired. The aim of this study was to analyze vaccine proteomes and identify target molecules common to allL. interrogansserovar Canicola vaccines which could be used to design anin vitropotency test. Initial analysis ofL. interrogansserovar Canicola vaccines (A to E) from different manufacturers, using theLimulusamebocyte lysate assay and silver-stained sodium dodecyl sulfate polyacrylamide gels, indicated that lipopolysaccharide was not present in all vaccines, preventing it from being a suitable target molecule. The protein contents of vaccines A to E were therefore determined by two-dimensional liquid chromatography mass spectrometry ([2D-LC/MS] 221 ± 31, 9 ± 8, 34 ± 4, 21 ± 5, and 34 ± 17 proteins [mean ± 1 standard deviation] found, respectively). The outer membrane protein LipL32 was established to be common to all and to be present at a significantly higher (P≤ 0.05) relative spectral abundance in a batch of vaccine which passed thein vivopotency test than in one which had failed. Further analysis using multiple reaction monitoring revealed that the concentration of the N terminus of LipL32 was significantly lower (P≤ 0.01) in failed batches (n= 2) of vaccine than in passed batches (n= 2); the concentration of the C terminus between the two batches was approximately the same. Anin vitro Leptospiravaccine potency test, based on N-terminal amino acid quantification of LipL32, was subsequently developed. |
| Databáze: | OpenAIRE |
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