Nondestructive Diagnostic Test for Nitrogen Nutrition of Grapevine (Vitis vinifera L.) Based on Dualex Leaf-Clip Measurements in the Field
| Autor: | Naïma Ben Ghozlen, Sébastien Debuisson, Charlotte Milhade, Mickaël Obert, Marine Le Moigne, Zoran G. Cerovic |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Nitrogen balance Nitrogen chemistry.chemical_element 01 natural sciences Chemistry Techniques Analytical Petiole (botany) chemistry.chemical_compound Dry weight Vitis Kjeldahl method 2. Zero hunger Crop yield 04 agricultural and veterinary sciences General Chemistry 15. Life on land Plant Leaves Horticulture chemistry Agronomy Chlorophyll Shoot 040103 agronomy & agriculture 0401 agriculture forestry and fisheries General Agricultural and Biological Sciences 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 63:3669-3680 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.5b00304 |
| Popis: | Crop nitrogen status is a major issue for crop yield and quality. It is usually assessed by destructive leaf or petiole tissue analysis. A quantitative nondestructive optical estimation of N sufficiency would be a great leap forward toward precision crop management. We therefore calibrated three optical indices against leaf nitrogen content: chlorophyll (Chl), epidermal flavonols, and the nitrogen balance index (NBI), which is the ratio of the former two indices. NBI was the best estimator of leaf N content measured by the Dumas or Kjeldahl method with a root-mean-square error smaller than 2 mg of N g(-1) dry weight, followed by Chl (3 mg g(-1)) and flavonols (4 mg g(-1)). This allowed us to propose the threshold values for the Dualex optical indices that characterize nitrogen supply to grapevines: the first is the threshold below which N supply to the vine can be considered deficient, and the second is the threshold above which N supply is excessive. For a putative optimal N content of 30 mg g(-1) < x < 40 mg g(-1), these thresholds are 30 μg cm(-2) < x < 40 μg cm(-2) for Chl and 11 < x < 18 for NBI at flowering. At bunch closure, for N thresholds of 22 < x < 32, Chl is 29 < x < 37 and NBI is 8 < x < 11, in respective units. These values should be verified and refined in the future for various growth regions and cultivars using the specified protocol. The sample size should be 36-60 leaves from a fixed node position, preferably node no. 5 from the tip of the shoot. An alternative to the use of the NBI would be to discard leaves that are not light exposed by checking their flavonol content and to deduce the N sufficiency directly from the Chl values. |
| Databáze: | OpenAIRE |
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