Adjuvant effect of recombinant interleukin-12 in the Nocardiosis formalin-killed vaccine of the amberjack Seriola dumerili
| Autor: | Kazuma Hayashi, Kazuhiro Shiozaki, Yutaka Takeuchi, Kyosuke Araki, Megumi Matsumoto, Hiroaki Suetake, Atsushi Yamamoto |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Fish Proteins medicine.medical_treatment Nocardia Infections chemical and pharmacologic phenomena Aquatic Science Nocardia 03 medical and health sciences Antigen Adjuvants Immunologic Immunity Formaldehyde medicine Environmental Chemistry Animals Amberjack biology Antibody titer 04 agricultural and veterinary sciences General Medicine biology.organism_classification Interleukin-12 Recombinant Proteins Perciformes 030104 developmental biology Vaccines Inactivated Immunology Humoral immunity Bacterial Vaccines 040102 fisheries Interleukin 12 biology.protein 0401 agriculture forestry and fisheries Antibody Adjuvant |
| Zdroj: | Fishshellfish immunology. 67 |
| ISSN: | 1095-9947 |
| Popis: | Nocardiosis causes serious economic damage in the fish farming of Japanese yellowtail fish. Nocardia seriolae identified as pathogenic bacterium is an intracellular-pathogen. In general, induction of cell-mediated immunity (CMI) is effective in infection defense against intracellular-pathogen. However, the conventional formalin-killed N. seriolae (FKC) vaccine induces humoral immunity. Interleukin-12 (IL-12) is Th1 type heterodimeric cytokine and induces cell differentiation in mammals. Our previous study showed that recombinant amberjack IL-12 has a role in CMI induction in vitro and could be a possible CMI inducing adjuvant. However, its adjuvant effect of fish IL-12 was not studied. In the present study, six types of amberjack recombinant IL-12 (rIL-12) were mixed and injected into amberjack with FKC. Firstly, we analyzed Th1- and Th2- related gene expression and monitored Th1/Th2 status followed by investigation of antibody titer. As a result, Th1-type immunity was induced in FKC + rIL-12 vaccinated fish. Secondly, we checked Th1/Th2 status of vaccinated fish after 10 days of N. seriolae infection using the expression of related genes. High T-bet/GATA-3 ratio was observed in FKC + rIL-12 vaccinated fish, suggesting that Th1 cells possesing antigen memory were induced against N. seriolae infection. Finally, the survival rate in challenge test showed that 88% of FKC + rIL-12 vaccinated fish was survived at 34 days after N. seriolae injection whereas PBS (control) and FKC only were exterminated. These result suggest that i) rIL-12 is viable CMI inducible adjuvant and ii) production of Th1 cells having antigen memory resulting from activation of IL-12 signaling pathway is important for defense against N. seriolae infection. |
| Databáze: | OpenAIRE |
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