Thiol-Ene Alginate Hydrogels as Versatile Bioinks for Bioprinting
| Autor: | Andrea Roberto Calore, A. Tessa ten Cate, Huey Wen Ooi, Carlos Mota, Lorenzo Moroni, Matthew B. Baker |
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| Přispěvatelé: | RS: MERLN - Complex Tissue Regeneration (CTR), CTR |
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Polymers and Plastics STRATEGIES Alginates MATRIX ELASTICITY Bioengineering Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Cell Line CONSTRUCTS chemistry.chemical_compound Mice Tissue engineering FUTURE CHEMISTRY Materials Chemistry Animals BIOMATERIALS Ene reaction Acrylate Sulfur Compounds Bioprinting Dithiol Biomaterial BIOFABRICATION Hydrogels Fibroblasts 021001 nanoscience & nanotechnology CELL VIABILITY Norbornanes 0104 chemical sciences Cross-Linking Reagents chemistry TISSUE Self-healing hydrogels MORPHOLOGY Calcium Ink Alginate hydrogel 0210 nano-technology Biofabrication |
| Zdroj: | Biomacromolecules, 19(8), 3390-3400. American Chemical Society |
| ISSN: | 1526-4602 1525-7797 |
| Popis: | [Image: see text] Bioprinting is a powerful technique that allows precise and controlled 3D deposition of biomaterials in a predesigned, customizable, and reproducible manner. Cell-laden hydrogel (“bioink”) bioprinting is especially advantageous for tissue engineering applications as multiple cells and biomaterial compositions can be selectively dispensed to create spatially well-defined architectures. Despite this promise, few hydrogel systems are easily available and suitable as bioinks, with even fewer systems allowing for molecular design of mechanical and biological properties. In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol–ene cross-linking mechanism that avoids acrylate kinetic chain formation. The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium. The low concentrations of alginate needed ( |
| Databáze: | OpenAIRE |
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