Zika Virus RNA Replication and Persistence in Brain and Placental Tissue
| Autor: | Yokabed Ermias, Roosecelis B Martines, Joy Gary, Amy J. Lambert, Edgar Alberto Parra Saad, Jana M. Ritter, Wun-Ju Shieh, M. Kelly Keating, Atis Muehlenbachs, Sarah Reagan-Steiner, Robert S. Lanciotti, Gillian Hale, Fernando Bolaños, Demi B. Rabeneck, Titilope Oduyebo, Lindsey B.C. Estetter, Julu Bhatnagar, Dana Meaney-Delman, Sherif R. Zaki, Tadaki Suzuki |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microcephaly Epidemiology viruses vector-borne infections lcsh:Medicine Virus Replication in-situ hybridization Zika virus Pregnancy Pregnancy Complications Infectious biology Reverse Transcriptase Polymerase Chain Reaction Zika Virus Infection Infectious Diseases Real-time polymerase chain reaction medicine.anatomical_structure RNA Viral Female paraffin-embedded tissues Microbiology (medical) Adult replication placenta Adolescent brain RT-PCR Zika Virus RNA Replication and Persistence in Brain and Placental Tissue lcsh:Infectious and parasitic diseases 03 medical and health sciences Young Adult Fetus formalin-fixed Placenta medicine Humans lcsh:RC109-216 Tropism Research lcsh:R Infant biology.organism_classification medicine.disease Virology Infectious Disease Transmission Vertical Abortion Spontaneous 030104 developmental biology Viral replication |
| Zdroj: | Emerging Infectious Diseases, Vol 23, Iss 3, Pp 405-414 (2017) Emerging Infectious Diseases |
| ISSN: | 1080-6059 1080-6040 |
| Popis: | Zika virus is causally linked with congenital microcephaly and may be associated with pregnancy loss. However, the mechanisms of Zika virus intrauterine transmission and replication and its tropism and persistence in tissues are poorly understood. We tested tissues from 52 case-patients: 8 infants with microcephaly who died and 44 women suspected of being infected with Zika virus during pregnancy. By reverse transcription PCR, tissues from 32 (62%) case-patients (brains from 8 infants with microcephaly and placental/fetal tissues from 24 women) were positive for Zika virus. In situ hybridization localized replicative Zika virus RNA in brains of 7 infants and in placentas of 9 women who had pregnancy losses during the first or second trimester. These findings demonstrate that Zika virus replicates and persists in fetal brains and placentas, providing direct evidence of its association with microcephaly. Tissue-based reverse transcription PCR extends the time frame of Zika virus detection in congenital and pregnancy-associated infections. |
| Databáze: | OpenAIRE |
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