1H-NMR metabolomics reveals a multitarget action of Crithmum maritimum ethyl acetate extract in inhibiting hepatocellular carcinoma cell growth
| Autor: | Francesca Castellaneta, Antonio Mazzocca, Chiara Roberta Girelli, Gianluigi Cesari, Davide Gnocchi, Francesco Paolo Fanizzi, Carlo Sabbà, Laura Del Coco |
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| Přispěvatelé: | Gnocchi, Davide, Del Coco, Laura, Girelli, Chiara Roberta, Castellaneta, Francesca, Cesari, Gianluigi, Sabbà, Carlo, Fanizzi, Francesco Paolo, Mazzocca, Antonio |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Multidisciplinary Biochemical networks biology Hepatocellular carcinoma Cell growth Cholesterol Science Pharmacology biology.organism_classification Cytostasis Warburg effect Article chemistry.chemical_compound NMR spectroscopy Biosynthesis chemistry Preclinical research Crithmum Medicine Polyunsaturated fatty acid Phosphocholine |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
| Popis: | Hepatocellular carcinoma (HCC) is nowadays the sixth cause of tumour-related deceases worldwide, estimated to become the third in Western countries by 2030. New drugs for HCC treatment still have many adverse effects. Several lines of evidence indicate that plant metabolites offer concrete opportunities for developing new therapeutic strategies for many diseases, including cancer. We previously reported that ethyl acetate extract of a spontaneous edible plant harvested in Apulia, Crithmum maritimum, significantly inhibited cell growth in HCC cells. By 1H-NMR spectroscopy, here we show that Crithmum maritimum ethyl acetate extract counteracts the Warburg effect, by reducing intracellular lactate, inhibits protein anabolism, by decreasing amino acid level, and affects membrane biosynthesis by lowering choline and phosphocholine. Also, we observed an effect on lipid homeostasis, with a reduction in triglycerides, cholesterol, monounsaturated fatty acids (MUFA), and diunsaturated fatty acids (DUFA), and an increase in polyunsaturated fatty acids (PUFA). Taken together, these data demonstrate that Crithmum maritimum-induced cytostasis is exerted through a multi-effect action, targeting key metabolic processes in HCC cells. Overall, our findings highlight the role of Crithmum maritimum as a promising tool for the prevention and the improvement of the therapeutic options for HCC and other types of tumours. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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