An insight into the antibiofilm properties of Costa Rican stingless bee honeys
| Autor: | Reindert Nijland, H. C. Quarles van Ufford, L.G. Zamora, Piet C. Aerts, M.L. Arias, C. J. Beukelman, A. J. J. van den Berg |
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| Rok vydání: | 2017 |
| Předmět: |
Costa Rica
0106 biological sciences Staphylococcus aureus Nursing (miscellaneous) Stingless bee medicine.drug_class medicine.medical_treatment Antibiotics Microbial Sensitivity Tests Melipona beecheii medicine.disease_cause 01 natural sciences Microbiology 030207 dermatology & venereal diseases 03 medical and health sciences Minimum inhibitory concentration 0302 clinical medicine Vancomycin Stingless bee honey medicine Animals Deoxyribonucleases Protease biology Biofilm food and beverages Honey Bees biology.organism_classification Antimicrobial Antibiofilm Anti-Bacterial Agents Laboratorium voor Phytopathologie 010602 entomology Biofilms Amylases Laboratory of Phytopathology Ampicillin Tetragonisca angustula Fundamentals and skills EPS |
| Zdroj: | Journal of Wound Care 26 (2017) 4 Journal of Wound Care, 26(4), 168-177 |
| ISSN: | 2052-2916 0969-0700 |
| Popis: | Objective: There is an increasing search for antibiofilm agents that either have specific activity against biofilms or may act in synergy with antimicrobials. Our objective is to examine the the antibiofilm properties of stingless bee honeys. Method: Meliponini honeys from Costa Rica were examined along with Medihoney as a reference. All honeys were submitted to a screening composed of minimum inhibitory concentration, inhibition of biofilm formation and biofilm destruction microplate-based assays against a Staphylococcus aureus biofilm forming strain. Dialysis led to the isolation of an antibiofilm fraction in Tetragonisca angustula honeys. The honey antibiofilm fraction was evaluated for protease activity and for any synergistic effect with antibiotics on a Staphylococcus aureus biofilm. The active fraction was then separated through activity guided isolation techniques involving SDS-PAGEs, anion exchange and size exclusion fast protein liquid chromatographies. The fractions obtained and the isolated antibiofilm constituents were tested for amylase and DNase activity. Results: A total of 57 Meliponini honeys from Costa Rica were studied in this research. The honeys studied belonged to the Tetragonisca angustula (n=36) and Melipona beecheii (n=21) species. Costa Rican Tetragonisca angustula honeys can inhibit the planktonic growth, biofilm formation, and are capable of destroying a Staphylococcus aureus biofilm. The antibiofilm effect was observed in the protein fraction of Tetragonisca angustula honeys. The biofilm destruction proteins allowed ampicillin and vancomycin to recover their antimicrobial activity over a Staphylococcus aureus biofilm. The antibiofilm proteins are of bee origin, and their activity was not due to serine, cysteine or metalloproteases. There were 2 proteins causing the antibiofilm action; these were named the Tetragonisca angustula biofilm destruction factors (TABDFs). TABDF-1 is a monomeric protein of approximately 50kDa that is responsible of the amylase activity of Tetragonisca angustula honeys. TABDF-2 is a protein monomer of approximately 75kDa. Conclusion: Tetragonisca angustula honeys from Costa Rica are a promising candidate for research and development of novel wound dressings focused on the treatment of acute and chronic Staphylococcus aureus biofilm wound infections. |
| Databáze: | OpenAIRE |
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